產(chǎn)品編號 | bs-18434R-Gold |
英文名稱 | Rabbit Anti-LSM10/Gold Conjugated antibody |
中文名稱 | 膠體金標記的LSM10相關蛋白抗體 |
別 名 | LSM 10; LSM10 U7 small nuclear RNA associated; MGC15749; MST074; LSM10_HUMAN; MSTP074; U7 snRNA associated Sm like protein LSm10; U7 snRNP specific Sm like protein LSM10. |
規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
說 明 書 | 100ul(10nm 15nm 35nm) |
研究領域 | 細胞生物 結合蛋白 表觀遺傳學 |
抗體來源 | Rabbit |
克隆類型 | Polyclonal |
交叉反應 | (predicted: Human, Mouse, Rat, Cow, Horse, Rabbit, ) |
產(chǎn)品應用 | IEM=1:20-200 ICA=1:20-200 ChIP=1:20-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
分 子 量 | 14kDa |
性 狀 | Lyophilized or Liquid |
濃 度 | 0.4mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human LSM10 |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
儲 存 液 | 0.02M TBS(pH8.2) with 1% BSA, 0.03% Proclin300. |
保存條件 | Store at 2-8 oC for 3-6 months. Avoid repeated freeze/thaw cycles. |
產(chǎn)品介紹 |
background: LSM10 is a 14 kDa polypeptide that is a member of the Sm/Lsm protein family. Like U7 snRNA, LSM10 is enriched in Cajal or coiled bodies (CBs) in the nucleus. LSM10 associates with the U7 snRNP and this interaction is dictated by the special Sm binding site of U7 snRNA. Function: Appears to function in the U7 snRNP complex that is involved in histone 3'-end processing. Increases U7 snRNA levels but not histone 3'-end pre-mRNA processing activity, when overexpressed. Required for cell cycle progression from G1 to S phases. Binds specifically to U7 snRNA. Binds to the downstream cleavage product (DCP) of histone pre-mRNA in a U7 snRNP dependent manner. Subunit: Component of the heptameric ring U7 snRNP complex, or U7 Sm protein core complex, at least composed of LSM10, LSM11, SNRPB, SNRPD3, SNRPE, SNRPF, SNRPG and U7 snRNA. Formation of the U7 snRNP is an ATP-dependent process mediated by a specialized SMN complex containing at least the Sm protein core complex and additionally, the U7-specific LSM10 and LSM11 proteins. Interacts with CLNS1A and SMN. Subcellular Location: Nuclear Post-translational modifications: Not methylated. Methylation is not necessary for interaction with SMN. Similarity: Belongs to the snRNP Sm proteins family. Database links: Entrez Gene: 84967 Human Entrez Gene: 116748 Mouse SwissProt: Q969L4 Human SwissProt: Q8QZX5 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
1、抗體溶解方法 | |
2、抗體修復方式 | |
3、常用試劑的配制 | |
4、免疫組化操作步驟 | |
5、免疫組化問題解答 | |
6、Western Blotting 操作步驟 | |
7、Western Blotting 問題解答 | |
8、關于肽鏈的設計 | |
9、多肽的溶解與保存 | |
10、酶標抗體效價測定程序 | |