| 產(chǎn)品編號 | bs-14601R-HRP |
| 英文名稱1 | Rabbit Anti-ENTPD4/HRP Conjugated antibody |
| 中文名稱 | 辣根過氧化物酶標記的ENTPD4蛋白抗體 |
| 別 名 | Apyrase lysosomal; Ectonucleoside triphosphate diphosphohydrolase 4; ENTPD 4; Golgi luminal UDPase; Guanosine diphosphatase like protein; KIAA0392; LALP70; ENTP4_HUMAN; LAP70; LYSAL1; Lysosomal apyrase like 1; Lysosomal apyrase like protein of 70 kDa; NTPDase 4; UDPase; Uridine diphosphatase |
| 規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 研究領(lǐng)域 | 腫瘤 細胞生物 信號轉(zhuǎn)導(dǎo) 新陳代謝 表觀遺傳學(xué) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | (predicted: Human, Mouse, Rat, Dog, Pig, Cow, Horse, ) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000 ELISA=1:100-1000 IHC-P=1:50-200 IHC-F=1:50-200 ICC=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 70kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ENTPD4 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產(chǎn)品介紹 |
background: This gene encodes a member of the apyrase protein family. Apyrases are enzymes that catalyze the hydrolysis of nucleotide diphosphates and triphosphates in a calcium or magnesium-dependent manner. The encoded protein is an endo-apyrase and may play a role in salvaging nucleotides from lysosomes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and these isoforms may differ in divalent cation dependence and substrate specificity. [provided by RefSeq, Sep 2011] Function: ENTPD4 (Ectonucleoside triphosphate diphosphohydrolase 4) preferentially hydrolyzes nucleoside 5'-diphosphates. Nucleoside 5'-triphosphates are hydrolyzed only to a minor extent. The order of activity with different substrates is UDP >> GDP = CDP = TDP. AMP, ADP, ATP and UMP are not substrates. There are two named isoforms. The preferred substrates for isoform 2 are CTP, UDP, CDP, GTP and GDP, while isoform 1 utilizes UTP and TTP. Subcellular Location: Golgi apparatus membrane; Multi-pass membrane protein. Cytoplasmic vesicle, autophagosome membrane; Multi-pass membrane protein. Note: Localizes in the Golgi and autophagic vacuoles/lysosomes. Tissue Specificity: Ubiquitous. Highest expression in testis and lowest in bladder. Similarity: Belongs to the GDA1/CD39 NTPase family. Database links: Entrez Gene: 9583 Human Entrez Gene: 67464 Mouse Omim: 607577 Human SwissProt: Q9Y227 Human SwissProt: Q9DBT4 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
