| 產品編號 | bs-11558R-RBITC |
| 英文名稱1 | Rabbit Anti-GCNF/RBITC Conjugated antibody |
| 中文名稱 | 羅丹明（RBITC）標記的生殖細胞核因子GCNF/維甲酸受體相關睪丸受體抗體 |
| 別 名 | GCNF 1; GCNF; GCNF1; Germ cell nuclear factor; hGCNF; hRTR; NR 61; NR61; NR6A1; NR6A1_HUMAN; Nuclear receptor subfamily 6 group A member 1; Orphan nuclear receptor NR6A1; Retinoic acid receptor related testis associated receptor; Retinoid receptor related testis specific receptor; Retinoid receptor-related testis-specific receptor; RTR. |
| 規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 研究領域 | 細胞生物 發(fā)育生物學 神經生物學 信號轉導 干細胞 轉錄調節(jié)因子 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | (predicted: Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, ) |
| 產品應用 | ICC=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 54kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GCNF (251-350aa) |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產品介紹 |
background: Germ cell nuclear factor (GCNF) is an orphan member of the nuclear receptor gene superfamily that influences neurogenesis and germ cell development. GCNF can homodimerize and bind DNA. GCNF regulates paracrine interaction between the oocyte and somatic cells by regulating the expression of BMP-15 and GDF-9, to affect female fertility. GCNF is present in spermatocytes and round spermatids of adult male mouse testis; northern blot and ribonuclease protection assays have shown GCNF is predominant in the testis. The gene expresses three alternatively spliced transcript variants. Function: Orphan nuclear receptor. Binds to a response element containing the sequence 5'-TCAAGGTCA-3'. May be involved in the regulation of gene expression in germ cell development during gametogenesis. Subunit: Homodimer. Interacts with UIMC1 Subcellular Location: Nucleus. Tissue Specificity: Shows highest expression in the germ cells of the adult testis. Similarity: Belongs to the nuclear hormone receptor family. NR6 subfamily. Contains 1 nuclear receptor DNA-binding domain. Database links: Entrez Gene: 2649 Human Entrez Gene: 14536 Mouse Omim: 602778 Human SwissProt: Q15406 Human SwissProt: Q64249 Mouse Unigene: 586460 Human Unigene: 439703 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
