| 產(chǎn)品編號 | IHC0318H |
| 英文名稱 | Human AIF Ready-To-Use IHC Kit |
| 中文名稱 | 人凋亡誘導(dǎo)因子即用型免疫組化試劑盒 |
| 別 名 | AIF, AIFM1, AIFsh2, CMT2D, CMTX4, COWCK, COXPD6, DFNX5, Hq, NADMR, NAMSD, PDCD8 |
| 保存條件 | Please store components at the temperatures indicated on the individual tube labels. The kit is stable for 6 months from the date of receipt. |
| 產(chǎn)品介紹 |
Apoptosis Inducing Factor (AIF) causes chromatin condensation and DNA fragmentation. AIF was recently identified, and cloned. Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of caspase family, caspase activated DNase, and several novel proteins. Like the critical molecules, cytochrome c and caspase-9, in apoptosis, AIF localizes in mitochondria. AIF translocates to the nucleus when apoptosis is induced and induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. AIF induces chromatin condensation and large scale DNA fragmentation, which are the hallmarks of apoptosis, of the isolated nucleus and the nucleus in live cells by microinjection and apoptosis stimuli. AIF is highly conserved between human and mouse and widely expressed. Mutations in the AIF gene cause combined oxidative phosphorylation deficiency 6, which results in a severe mitochondrial encephalomyopathy. Alternative splicing results in multiple transcript variants of AIF and a related pseudogene has been identified on chromosome 10. |
| 產(chǎn)品圖片 |
Immunohistochemical analysis of paraffin embedded human kidney tissue slide using IHC0318H (Human AIF Kit).
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Immunohistochemical analysis of paraffin embedded human colon cancer tissue slide using IHC0318H (Human AIF Kit).
Immunohistochemical analysis of paraffin embedded human testis tissue slide using IHC0318H (Human AIF Kit).
Immunohistochemical analysis of paraffin embedded human hepatocellular carcinoma tissue slide using IHC0318H (Human AIF Kit).
Immunohistochemical analysis of paraffin embedded human liver tissue slide using IHC0318H (Human AIF Kit).
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
