| 產(chǎn)品編號 | IHC0308R |
| 英文名稱 | Rat Histone H2A.X Ready-To-Use IHC Kit |
| 中文名稱 | 大鼠組蛋白H2AX即用型免疫組化試劑盒 |
| 別 名 | gammaH2ax, H2A.X, H2A/X, Hist5-2ax, AW228881, H2A histone family member X, H2A.FX, H2A.X, H2a/x, H2AFX, H2AX, H2AX histone, H2AX_HUMAN, Hist5.2ax, Histone 2A, Histone 2AX, Histone H2A.X, Histone H2AX, RGD1566119 |
| 保存條件 | Please store components at the temperatures indicated on the individual tube labels. The kit is stable for 6 months from the date of receipt. |
| 產(chǎn)品介紹 |
Histone H2A.X (H2AX) is a member of the histone H2A family which is one of the four core histones making up the nucleosome core particle. In eukaryotes, DNA double strand breaks (DSBs) have been shown to trigger the phosphorylation of serine 139 at the carboxy terminus of histone H2AX resulting in gamma-H2AX. The phosphorylation of H2AX can be detected by Western blotting or immunofluorescence, revealing the frequency of DSBs. The phosphatidylinositol 3-kinases have been implicated in H2AX phosphorylation, but it is unclear if ATM is the primary H2AX kinase or if other members of the family such as DNA-PK and ATR contribute in a similar manner. Structurally, H2A.x contains 143 amino acid residues. Histone H2A.X is considered a basal histone, being synthesized in G1 as well as in S-phase, and its mRNA contains polyA addition motifs and a polyA tail along with the conserved stem-loop and U7 binding sequences involved in the processing and stability of replication type histone mRNAs. There are two forms of Histone H2A.X mRNA, one about 1600 bases long and contains polyA; the other about 575 bases long, lacking polyA. The short form behaves as a replication type histone mRNA, while the longer behaves as a basal type histone mRNA. Histone H2A.X maps to the 11q23.2-q23.3 region of the human chromosome. Histone H2A.x contributes to histone-formation and therefore the structure of DNA. Histone H2A variant H2A.x specifically regulates the interaction of MDC1 (mediator of DNA damage checkpoint protein 1), a DNA repair protein to the sites of DNA damage. |
| 產(chǎn)品圖片 |
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Immunohistochemical analysis of paraffin embedded rat colon tissue slide using IHC0308R (Rat Histone H2A.X Kit).
Immunohistochemical analysis of paraffin embedded rat testis tissue slide using IHC0308R (Rat Histone H2A.X Kit).
Immunohistochemical analysis of paraffin embedded rat lung tissue slide using IHC0308R (Rat Histone H2A.X Kit).
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
