| 產品編號 | IHC0307R |
| 英文名稱 | Rat Beta tubulin Ready-To-Use IHC Kit |
| 中文名稱 | 大鼠微管蛋白β tubulin/Tubulin β即用型免疫組化試劑盒 |
| 別 名 | 2310057H16Rik, 2410129E14Rik, 2810484G07Rik, 3200002H15Rik, AA408537, AI325297, AI596182, B130022C14Rik, bA506K6.1, bA631M21.2, BB220206, beta-4, beta-5, brdp, CDCBM, CDCBM1, CDCBM5, CDCBM6, CFEOM3, CFEOM3A, CSCSC1, DYT4, FEOM3, HsT1601, M(beta)1, M(beta) |
| 保存條件 | Please store components at the temperatures indicated on the individual tube labels. The kit is stable for 6 months from the date of receipt. |
| 產品介紹 |
Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. Beta-III tubulin is primarily expressed in neurons and may be involved in neurogenesis, axon guidance and maintenance. Mutations in the beta tubulin gene are the cause of congenital fibrosis of the extraocular muscles type 3. Beta-III tubulin was also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues. Glutamate residues at the C-terminus of beta III tubulin can be glutamylated. The precise function of such modifications is unclear. Tubulin is phosphorylated on Ser-172 by CDK1 during cell cycle progression. Ser-172 phosphorylation inhibits tubulin incorporation into microtubules.Microtubules, the major cytoskeletal elements found in all eukaryotic cells, consist of Tubulin, which is a dimer of two 55 kDa subunits: alpha and Beta. Microtubules play key roles in chromosome segregation in mitosis, intracellular transport, ciliary and flagellar bending, and structural support of the cytoskeleton. Because beta-tubulin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting. |
| 產品圖片 |
Immunohistochemical analysis of paraffin embedded rat cerebellum tissue slide using IHC0307R (Rat Beta tubulin Kit).
Immunohistochemical analysis of paraffin embedded rat brain tissue slide using IHC0307R (Rat Beta tubulin Kit).
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
