| 產品編號 | bsm-63283R |
| 英文名稱 | phospho-CHEK1 (S280) Recombinant Rabbit mAb |
| 中文名稱 | 磷酸化細胞周期檢測點激酶1重組兔單抗 |
| 別 名 | Checkpoint kinase 1; Chek1; Chk1; rad27. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | 5D11 |
| 交叉反應 | Human |
| 產品應用 | WB=1:1000-1:2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 54 kDa |
| 檢測分子量 | 54 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human Chk1 around the phosphorylation site of S280: VT-pS-GG |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest and activation of DNA repair in response to the presence of DNA damage or unreplicated DNA. SWISS: O14757 Gene ID: 1111 Chk1作為Cdks的調節(jié)參與細胞周期調節(jié)過程,是生物進化過程中非常保守的蛋白激酶，在DNA損傷引起的細胞周期檢測點調節(jié)中有著非常重要的作用.在細胞周期檢測點信號傳導通路中，Chkl和Chk2起著重要作用，主要參與G2/M期細胞周期檢測點信號傳導. |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
