| 產(chǎn)品編號 | bsm-55545R |
| 英文名稱 | Myeloperoxidase Recombinant Rabbit mAb |
| 中文名稱 | 髓過氧化物酶重組兔單抗 |
| 別 名 | MPO; myeloperoxidase |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | SB135 |
| 交叉反應 | Mouse,Rat |
| 產(chǎn)品應用 | IHC-P=1:200-800,IHC-F=1:200-800,IF=1:200-800
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | kDa |
| 檢測分子量 | |
| 性 狀 | Liquid |
| 免 疫 原 | KLH conjugated synthetic peptide derived from Mouse MPO |
| 亞 型 | IgG |
| 純化方法 | Affinity Purification |
| 緩 沖 液 | PBS with 100 μg/ml BSA,0.15% ProClin300 and 50% glycerol. |
| 保存條件 | Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity. SWISS: P11247 Gene ID: 17523 髓過氧化物酶（MPO）是一種過氧化物酶，由17號染色體上的MPO基因編碼。MPO在中性粒細胞中表達得最多，并產(chǎn)生次氯酸以進行抗菌活動，其鈉鹽是漂白劑中的化學物質(zhì)。它是一種溶酶體蛋白，儲存在中性粒細胞的嗜氮顆粒中，并在脫顆粒時釋放到細胞外空間。MPO在急性骨髓性白血病、粒細胞性白血病、單核細胞性白血病、成髓細胞瘤和其它造血系統(tǒng)疾病的成髓細胞和未成熟骨髓細胞中表達，在某些肺癌和卵巢癌也可見表達。 |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Myeloperoxidase Monoclonal Antibody, Unconjugated (bsm-55545R) at 1:500 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Myeloperoxidase Monoclonal Antibody, Unconjugated (bsm-55545R) at 1:500 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
