| 產(chǎn)品編號 | bsm-60864M |
| 英文名稱 | Mouse Anti-Benzoyllysine antibody |
| 中文名稱 | 苯甲?；嚢彼?苯甲?；嚢彼釂慰寺】贵w |
| 別 名 | Benzoylated Lysine; Benzoyllysine; benzyl-lysine; benzyl Lys; Kbz; 賴氨酸苯甲酰化; 苯甲?；揎椯嚢彼?/td> |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號 | 9B7 |
| 交叉反應(yīng) | Human,Mouse,Rat |
| 產(chǎn)品應(yīng)用 | WB=1:500-1000,IHC-P=1:200-1000,IHC-F=1:200-1000,IF=1:200-1000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | benzoylated BSA |
| 亞 型 | IgG |
| 純化方法 | 免疫親和層析純化 |
| 緩 沖 液 | PBS, Glycerol, BSA. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Lysine benzoyllysine is a newly-identified histone post-translational modification by integrated proteomic approaches and elaborate biochemistry analyses. It has been shown that lysine benzoylation is an evolutionarily conserved PTM in multiple species of both prokaryotes and eukaryotes. 賴氨酸苯甲?；且环N進化保守的修飾，在很多原核和真核生物中都有表達。 |
| 產(chǎn)品圖片 |
Sealing solution and concentration: 5% NFDM/TBST
First antibody dilution: 1:1000
Incubation conditions for primary antibody: 2 hours at room temperature
Secondary antibody: Goat Anti Mouse IgG H&L (HRP)
Cracking liquid: HeLa, N2a, HepG2
Protein loading amount: 20 μ G
Exposure time: 60 seconds
Theoretical molecular weight: Multiple kDa
Actual molecular weight: Multiple kDa
Tissue: human placenta
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: SP Kit(Mouse)(sp-0024)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bsm-60864M
Tissue: human testis
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: SP Kit(Mouse)(sp-0024)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bsm-60864M
Tissue: rat brain
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: SP Kit(Mouse)(sp-0024)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bsm-60864M
Tissue: mouse liver
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: SP Kit(Mouse)(sp-0024)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bsm-60864M
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
