| 產(chǎn)品編號 | D50701 |
| 英文名稱 | Valspodar |
| 中文名稱 | 伐司撲達 |
| 別 名 | PSC 833; P-glycoprotein inhibitor; 3'-Keto-bmt(1)-Val(2)-cyclosporin a; Cyclosporin A, 6-((R-(E))-6,7-didehydro-N,4-dimethyl-3-oxo-L-2-aminooctanoic acid)-7-L-valine-; 戊司泊達; P-糖蛋白抑制劑; P糖蛋白抑制劑; |
| 克 隆 號 | |
| CAS | 21584-18-7 |
| 理論分子量 | kDa |
| 檢測分子量 | |
| 保存條件 | Store at -20℃ for 3 year(Powder); In DMSO or others solvent store at 2-4℃ for two weeks, at -20℃ for six months. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產(chǎn)品介紹 |
基本信息： CAS：121584-18-7 分子式：C36H70N4O5 分子量：1214.62 純度：99% 溶解性：DMSO (12 mg/mL, 9.88 mM;) 性狀：白色到米色粉末 產(chǎn)品簡介： 伐司樸達（Valspodar; PSC 833）是一種選擇性的 P-糖蛋白抑制劑，已被用作化學(xué)增敏劑用于實驗性癌癥的研究。 作用靶點：P-glycoprotein; 作用通路：Membrane Transporter/Ion Channel; 體外研究：Cancer 體外研究：Valspodar (PSC833) has no cytotoxicity effects at up to the concentration of 0.75μg/mL. Valspodar (0.25, 0.5 and 0.75μg/mL) and DOX-L are added to the DOX resistant cells, and cell kill efficacy of MDR cell type increases significantly when valspodar is administered alongside DOX-L. Valspodar 0.5 and 0.75μg/mL), in combination with all concentrations of DOX, are most toxic and kill more than 70% of the resistant cells. Pretreatment with PSC833 decreases the IC50 value of NSC 279836 in MDA-MB-435mdr cells to 0.4±0.02μM in MDR cells and almost completely reverses the resistance of MDR cells to NSC 279836. 體內(nèi)研究：valspodar (10mg/kg,o.p.) exhibits minimal blood-cell partitioning as reflected in its low mean blood-to-plasma ratio of approximately 0.52. Valspodar isplays properties of slow learance and a large volume of distribution. Valspodar shows properties of low hepatic extraction and ide distribution, similar to that of its structural analogue CsA. Preadministration of PSC833 to mice increases NSC 279836 fluorescent intensity in MDR tumor to 94% of that in the wild-type tumors. |
| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
