| 產(chǎn)品編號(hào) | bsm-60559M |
| 英文名稱 | CHEK2 Mouse mAb |
| 中文名稱 | 細(xì)胞周期檢測(cè)點(diǎn)激酶2單克隆抗體 |
| 別 名 | CHK2_HUMAN; Serine/threonine-protein kinase Chk2; EC:2.7.11.1; Serine/threonine-protein kinase Chk2; CDS1; CHK2; LFS2; RAD53; hCds1; HuCds1; PP1425; CHK2 checkpoint homolog; Cds1 homolog(Hucds1; hCds1); Checkpoint kinase 2; |
| 抗體來(lái)源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號(hào) | E3E7 |
| 交叉反應(yīng) | Human |
| 產(chǎn)品應(yīng)用 | WB=1:500-1:1000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 56 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 亞 型 | IgG2B/lambda |
| 純化方法 | affinity purified by Protein G |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012] Subcellular Location: Isoform 2: Nucleus. Note=Isoform 10 is present throughout the cell. Isoform 4: Nucleus. Isoform 7: Nucleus. Isoform 9: Nucleus. Isoform 12: Nucleus. Nucleus, PML body. Nucleus, nucleoplasm. Note=Recruited into PML bodies together with TP53. Tissue Specificity: High expression is found in testis, spleen, colon and peripheral blood leukocytes. Low expression is found in other tissues. Database links: Entrez Gene: 11200 Human SwissProt: O96017 Human 絲氨酸/蘇氨酸蛋白激酶，是檢測(cè)點(diǎn)介導(dǎo)的細(xì)胞周期阻滯、DNA修復(fù)激活和DNA雙鏈斷裂引起的細(xì)胞凋亡所必需的。在未受干擾的細(xì)胞周期中也可能負(fù)性調(diào)節(jié)細(xì)胞周期進(jìn)程。活化后，在共有序列[L-X-R-X-X-S/T]上優(yōu)先磷酸化許多效應(yīng)子。通過(guò)CDC25A、CDC25B和CDC25C的磷酸化調(diào)節(jié)細(xì)胞周期檢查點(diǎn)阻滯，抑制其活性。CDC25磷酸酶活性的抑制導(dǎo)致CDK-細(xì)胞周期蛋白復(fù)合物酪氨酸磷酸化的抑制性增加并阻斷細(xì)胞周期進(jìn)程。也可能磷酸化參與G2/M細(xì)胞周期阻滯的NEK6。通過(guò)BRCA2的磷酸化調(diào)節(jié)DNA修復(fù)，增強(qiáng)RAD51與染色質(zhì)的結(jié)合，通過(guò)同源重組促進(jìn)DNA修復(fù)。還通過(guò)轉(zhuǎn)錄因子FOXM1的磷酸化和激活刺激參與DNA修復(fù)的基因（包括BRCA2）的轉(zhuǎn)錄。 |
| 產(chǎn)品圖片 |
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:1000
Primary ab incubation condition: room temperature 2h
Secondary ab: Goat Anti-Mouse IgG H&L (HRP)
Lysate: HeLa, HCT-116, MOLT-4
Protein loading quantity: 20 μg
Exposure time: 30 s
Predicted MW: 62 kDa
Observed MW: 62 kDa
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