| 產(chǎn)品編號 | bsm-60201M |
| 英文名稱 | Mouse Anti-Propionyl-Histone H4 (Lys8) antibody |
| 中文名稱 | |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號 | E5F6 |
| 交叉反應(yīng) | Human,Mouse (predicted: Rat) |
| 產(chǎn)品應(yīng)用 | WB=1:500-1:1000,IHC-P=1:100-500,IHC-F=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 亞 型 | IgG |
| 純化方法 | Antigen affinity purification |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | 組蛋白受多種酶催化修飾，包括乙?；?、甲基化、磷酸化、泛素化等。賴氨酸丙?；↘prop）在結(jié)構(gòu)上與賴氨酸乙?；嗨疲且环N新發(fā)現(xiàn)的控制蛋白質(zhì)活性的可逆修飾。賴氨酸丙?；谠松锖驼婧松镏卸即罅看嬖?，并且已經(jīng)在包括組蛋白和非組蛋白底物（如p53）的廣泛蛋白質(zhì)中被發(fā)現(xiàn)。與組氨酸H3在Lys12位點的乙?；饔妙愃?，組蛋白H4在Lys8位點的丙酰化作用可能在表觀遺傳調(diào)控中發(fā)揮重要作用，包括染色質(zhì)重塑和轉(zhuǎn)錄調(diào)控。 |
| 產(chǎn)品圖片 |
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:1000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Mouse IgG H&L (HRP)
Lysate: (-) C2C12, (+) C2C12+Sodium butyrate (30mM, 4hr)
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 11 kDa
Observed MW: 11 kDa
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:1000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Mouse IgG H&L (HRP)
Lysate: (-) HeLa, (+) HeLa+Sodium butyrate (30mM, 4hr) +Trichostatin A (500ng/ml, 4hr)
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 11 kDa
Observed MW: 11 kDa
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