| 產(chǎn)品編號(hào) | D10692 |
| 英文名稱(chēng) | Recombinant Cholera Toxin B subunit |
| 中文名稱(chēng) | 重組霍亂毒素B亞基 |
| 別 名 | Cholera Toxin B subunit; B subunit Cholera Toxin; Beta subunit Cholera Toxin; CTxB; Choleragenoid; CTB; CHTB; 霍亂毒素亞型B(CTB); 霍亂毒素B亞基; 霍亂毒素B亞單位; |
| 克 隆 號(hào) | |
| CAS | 24730-31-2 |
| 理論分子量 | kDa |
| 檢測(cè)分子量 | |
| 保存條件 | Store at 2-8℃. DO NOT FREEZE. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產(chǎn)品介紹 |
基本信息： CAS：131096-89-4 分子量：11 kDa 種屬：Vibrio cholerae 來(lái)源：E. coli. 內(nèi)毒素：Less than 0.1EU/μg of rCTB as determined by LAL method. 純度：>98% by SDS-PAGE and HPLC analyses. 外觀：Sterile colorless liquid. 緩沖體系：Supplied as a 0.2μM filtered solution in 5mM PB, pH7.0, 75mM NaCl, with 50% glycerol. (Sodium azide free.) 描述：霍亂毒素B亞基 (Cholera Toxin B subunit）屬于毒素的AB5-亞單位家族。天然六聚體蛋白的分子量約為85 kDa，包含兩個(gè)亞單位。它由負(fù)責(zé)ADP核糖基化活性的單個(gè)a亞單位（~27.2 kDa）和五個(gè)B亞單位（~11.6 kDa）組成，排列為五聚環(huán)，具有明顯的5倍對(duì)稱(chēng)性，與細(xì)胞表面受體結(jié)合和酶組分的后續(xù)內(nèi)化（跨膜轉(zhuǎn)運(yùn)）有關(guān)。 背景資料： Cholera toxin is protein complex secreted by the bacterium Vibrio cholerae. CTX is responsible for the massive, watery diarrhea characteristic of cholera infection. The cholera toxin is an oligomeric complex made up of six protein subunits: a single copy of the A subunit (part A, enzymatic), and five copies of the B subunit (part B, receptor binding), denoted as AB5. Subunit B binds while subunit A activates the G protein which activates adenylate cyclase. The five B subunits - each weighing 11 kDa, form a five-membered ring. The A subunit which is 28 kDa, has two important segments. The A1 portion of the chain (CTA1) is a globular enzyme payload that ADP-ribosylates G proteins, while the A2 chain (CTA2) forms an extended alpha helix which sits snugly in the central pore of the B subunit ring. This structure is similar in shape, mechanism, and sequence to the heat-labile enterotoxin secreted by some strains of the Escherichia coli bacterium. |
