| 產(chǎn)品編號 | bsm-51567M |
| 英文名稱 | FUBP3 Mouse mAb |
| 中文名稱 | FUBP3單克隆抗體 |
| 別 名 | Far upstream element(FUSE) binding protein 3; Far upstream element binding protein 3; Far upstream element-binding protein 3; FBP3; FLJ25229; FUBP 3; FUBP3_HUMAN; FUSE binding protein 3; FUSE-binding protein 3; OTTHUMP00000022369. |
| 研究領(lǐng)域 | 腫瘤 細(xì)胞生物 信號轉(zhuǎn)導(dǎo) 轉(zhuǎn)錄調(diào)節(jié)因子 細(xì)胞分化 表觀遺傳學(xué) |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號 | Q2C12 |
| 交叉反應(yīng) | Human |
| 產(chǎn)品應(yīng)用 | WB=1:500-1000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 62 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant human FUBP3 (His-tagged). |
| 亞 型 | IgG1 |
| 純化方法 | affinity purified by Protein G |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Activation of FUSE, the far-upstream element, is required for the proper ex-pression of the mammalian gene c-Myc in undifferentiated cells. The binding of FBP (FUSE-binding protein or Far upstream element binding protein) to FUSE is necessary for c-Myc expression, indicating that FBP functions as a growth-dependent regulator of c-Myc expression. Isolated from proliferating HL60 cells, FBP, FBP2, and FBP3 comprise a family of single-stranded DNA-binding proteins that specifically bind to FUSE elements. The FBP transcription factors share a conserved central DNA-binding domain and show significant homology in their carboxyl-terminal activation domains. Expression of FBP is detected in undifferentiated cells and is substantially decreased following cellular differentiation. Function: May interact with single-stranded DNA from the far-upstream element (FUSE). May activate gene expression. Subunit: Interacts with YAF2 through the region encompassing the first and second zinc fingers. Component of the chromatin remodeling INO80 complex; specifically part of a complex module associated with the DBINO domain of INO80. Interacts with EED and EZH2; the interactions are indicative for an association with the PRC2/EED-EZH2 complex. Subcellular Location: Nucleus. Tissue Specificity: Detected in a number of cell lines. Post-translational modifications: Phosphorylated upon DNA damage, probably by ATM or ATR. Similarity: Contains 4 KH domains. SWISS: Q96I24 Gene ID: 8939 Database links: Entrez Gene: 8939 Human SwissProt: Q96I24 Human |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Hela cell lysates
Lane 2: U251 cell lysates
Primary: Anti-FUBP3 (bsm-51567M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 62 kD
Observed band size: 68 kD
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