| 產品編號 | D10376 |
| 英文名稱 | Lysozyme from chicken egg white |
| 中文名稱 | 溶菌酶 (≥40KU/mg, 進口) |
| 別 名 | Mucopeptide N-acetylmuramoylhydrolase, Muramidase; lyophilized powder, protein ≥90 %, ≥40,000 units/mg protein; 溶解酵素; 溶菌酶 來源于雞蛋白; 胞壁質酶; 粘肽 N-乙酰胞壁質水解酶; |
| 克 隆 號 | |
| CAS | 12650-88-3 |
| 理論分子量 | kDa |
| 檢測分子量 | |
| 保存條件 | Store at -20℃. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產品介紹 |
CAS號：12650-88-3 分子量：single-chain 14.3kDa MDL：MFCD00131557 級別/活性：BR,≥40,000 units/mg protein 純度：≥90% (HPLC) 外觀/性狀：lyophilized powder 酶活性定義：One unit will produce a ΔA450 of 0.001 per min at pH 6.24 at 25 °C, using a suspension of Micrococcus lysodeikticus as substrate, in a 2.6 mL reaction mixture (1 cm light path). Biochem/physiol Actions: Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan. Gram-negative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan. However, these cells may be hydrolyzed in the presence of EDTA that chelates metal ions in the outer bacterial membrane. The enzyme is active over a broad pH range (6.0 to 9.0). At pH 6.2, maximal activity is observed over a wider range of ionic strengths (0.02 to 0.100 M) than at pH 9.2 (0.01 to 0.06 M). L6876 (Sigma-Aldrich) |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
