| 產(chǎn)品編號 | D10379 |
| 英文名稱 | Lysostaphin from Staphylococcus simulans |
| 中文名稱 | 溶葡萄球菌酶 (≥90%, ≥3000 unit/mg) |
| 別 名 | Glycyl-glycine Endopeptidase; Lysostaphin from Staphylococcus simulans (≥90%, ≥3000 unit/mg); Lysostaphin from Staphylococcus simulans, Recombinant; Lysostaphin, Recombinant (Staphylococcus simulans); 溶葡球菌酶; 重組溶葡萄球菌酶; |
| 克 隆 號 | |
| CAS | 9011-93-2 |
| 理論分子量 | kDa |
| 檢測分子量 | |
| 保存條件 | Store at -20℃. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產(chǎn)品介紹 |
CAS號：9011-93-2 分子量：27kDa MDL：MFCD00131556 級別：BR 活性：≥3000 unit/mg solid 純度：≥90% (HPLC) 外觀/性狀：lyophilized powder 酶活性定義：One unit will reduce the turbidity (A620) of a suspension of Staphylococcus aureus cells from 0.250 to 0.125 in 10 min at pH 7.5 at 37°C in a 6.0 ml reaction mixture. 等電點(diǎn)：10.5-11.0。 酶活性最適pH范圍：10～11。 影響因素：羥汞苯甲酸（PCMB）、 苯甲磺酰氯（PMSF）、二硫蘇糖醇（DTT） 等在1mmol/L的條件下，對酶活力無明顯影響，Zn離子表現(xiàn)出某種程度的活化效應(yīng)；Ba離子、Mn離子、Ca離子、Mg離子和EDTA在1mmol/L的條件下，對溶葡球菌酶表現(xiàn)出一定程度的抑制作用 溶解性：可溶于50mmol/L Tris 緩沖液 來源：Recombinant, Expressed in E. coli. 用途：生化研究。專一降解金黃色葡萄球菌等細(xì)胞壁具有甘氨酸肽鍵結(jié)構(gòu)的細(xì)菌，通過裂解其細(xì)胞壁達(dá)到殺菌的目的。在pH值為4和5℃時(shí)高度穩(wěn)定。 產(chǎn)品描述：Lysostaphin from Staphylococcus simulans has been used in a study to assess molecular cloning, sequencing, and expression of lytM, a unique autolytic gene of Staphylococcus aureus. It has also been used in a study to investigate the sequence analysis of a Staphylococcus aureus gene encoding a peptidoglycan hydrolase activity. A protein complex with highly specific lytic activity against Staphylococcus species, including Staphylococcus aureus. Lysostaphin possesses a lytic action against Staphylococcus aureus, which is used to treat antibiotic-resistant staphylococcal infections. It has the properties of enzymes, such as glycylglycine endopeptidase, endo-β-N-acetyl glucosamidase and N-acetyl muramyl-L-alanine amidase. Lysostaphin is also used as a preservative in food industry and in clinical labs for rapid screening. The antimicrobial activity of lysostaphin, a metalloendopeptidase expressed by Staphylococcus simulans, is enhanced by binding to the C′ terminus of the membrane-associated Trap protein of Staphylococcus aureus. |
