| 產(chǎn)品編號 | bs-24328R |
| 英文名稱 | Rabbit Anti-lamin B1 antibody |
| 中文名稱 | 核纖層蛋白B(細胞核膜標志物)抗體 |
| 別 名 | LMNB1_HUMAN; Lamin-B1; LMNB1; LMN2; LMNB; LMN; ADLD; MCPH26; |
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Specific References (4) | bs-24328R has been referenced in 4 publications.
[IF=7.086] Xue Yang. et al. The heart as a target for deltamethrin toxicity: Inhibition of Nrf2/HO-1 pathway induces oxidative stress and results in inflammation and apoptosis. Chemosphere. 2022 Aug;300:134479 WB ; Japanese Quails.
[IF=5.59] Li, Jiayi. et al. Activation of the GPX4/TLR4 Signaling Pathway Participates in the Alleviation of Selenium Yeast on Deltamethrin-Provoked Cerebrum Injury in Quails. Mol Neurobiol. 2022 Mar;:1-16 WB ; Quails.
[IF=2.65] Xiaojing Qian. et al. Fisetin Ameliorates Diabetic Nephropathy-Induced Podocyte Injury by Modulating Nrf2/HO-1/GPX4 Signaling Pathway. EVID-BASED COMPL ALT. 2023 Jan 19;2023:9331546 WB ; Mouse.
[IF=2.375] Shu-wen Xu. et al. Cigarette smoke extract-induced inflammatory response via inhibition of the TFEB-mediated autophagy in NR8383 cells. EXP LUNG RES. 2023 Jan 13 WB ; Rat.
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| 研究領域 | 細胞生物 染色質(zhì)和核信號 信號轉導 細胞凋亡 細胞類型標志物 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human (predicted: Mouse,Rat,Pig,Sheep,Cow) |
| 產(chǎn)品應用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 64kDa |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Lamin B: 381-480/586 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. Alternative splicing results in transcript variants and a duplication of this gene is associated with autosomal dominant adult-onset leukodystrophy (ADLD). [provided by RefSeq, Oct 2010]. Function: Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. Subunit: Homodimer. Interacts with lamin-associated polypeptides IA, IB and 2. Subcellular Location: Nucleus inner membrane; Lipid-anchor; Nucleoplasmic side. Post-translational modifications: B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations. DISEASE: Defects in LMNB1 are the cause of leukodystrophy demyelinating autosomal dominant adult-onset (ADLD) [MIM:169500]. ADLD is a slowly progressive and fatal demyelinating leukodystrophy, presenting in the fourth or fifth decade of life. Clinically characterized by early autonomic abnormalities, pyramidal and cerebellar dysfunction, and symmetric demyelination of the CNS. It differs from multiple sclerosis and other demyelinating disorders in that neuropathology shows preservation of oligodendroglia in the presence of subtotal demyelination and lack of astrogliosis. Similarity: Belongs to the intermediate filament family. SWISS: P20700 Gene ID: 4001 Database links: Entrez Gene: 396223 Chicken Entrez Gene: 4001 Human Entrez Gene: 16906 Mouse Omim: 150340 Human SwissProt: P14731 Chicken SwissProt: P20700 Human SwissProt: P14733 Mouse Unigene: 89497 Human Unigene: 4105 Mouse Unigene: 11362 Rat 核膜標志物（Nuclear Envelope Marker） 核纖層蛋白(Lamin) 是緊貼核內(nèi)膜的一層厚度為20～50nm的纖維蛋白層或纖維網(wǎng)絡。核纖層與細胞質(zhì)骨架、核骨架連成一個整體，一般認為核纖層將核被膜和染色質(zhì)提供了結構支架。有學者研究認為：lamin蛋白與細胞凋亡及衰老有關聯(lián)，它包括：核纖層蛋白A、核纖層蛋白B、核纖層蛋白C幾個不同亞型的蛋白。 |
| 產(chǎn)品圖片 |
Sample:
Lane 1: U937 (Human) Cell Lysate at 30 ug
Lane 2: Jurkat (Human) Cell Lysate at 30 ug
Lane 3: Molt-4 (Human) Cell Lysate at 30 ug
Lane 4: Hela (Human) Cell Lysate at 30 ug
Lane 5: U2os (Human) Cell Lysate at 30 ug
Primary: Anti-Lamin B (bs-24328R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 64 kD
Observed band size: 64 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
