| 產(chǎn)品編號(hào) | C7102 |
| 英文名稱 | HAT Supplement (50×) |
| 中文名稱 | HAT培養(yǎng)基添加劑 (50×) |
| 別 名 | HAT Media Supplement (50×) Hybri-Max?; H0262 (Sigma-Aldrich); HAT Media Supplement(50×); HAT Supplement, Hypoxanthine-Aminopterin-Thymidin (HAT) media supplement HAT補(bǔ)充劑; 次黃嘌呤氨基蝶呤胸苷（HAT）培養(yǎng)基補(bǔ)充劑; HAT添加劑; |
| 克 隆 號(hào) | |
| CAS | |
| 理論分子量 | kDa |
| 檢測(cè)分子量 | |
| 保存條件 | Store at -20℃. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產(chǎn)品介紹 |
HAT Supplement is a liquid mixture of sodium hypoxanthine, aminopterin and thymidine. After dilution, HAT is suitable for use as a post-fusion selective medium to eliminate unfused or self-fused HGPRT-myeloma cells. γ-irradiated, lyophilized powder, BioXtra, suitable for hybridoma; Application: HAT Media Supplement (50×) Hybri-Max? has been used: ? in the selection and validation of hypoxanthine-guanine phosophoribosyltransferase (HPRT)-deficient clones. ? in the generation of mAbs (monoclonal antibodies). ? in the luciferase-based bioassay (LUC) assay. Reconstitution: Reconstitute contents of vial with 10 ml sterile cell culture medium. Stock solution is sufficient to prepare 500 ml medium. Final working concentration: 100 μM hypoxanthine, 0.4 μM aminopterin, 16 μM thymidine. |
