產(chǎn)品編號 | D50381 |
英文名稱 | AEBSF hydrochloride |
中文名稱 | 4-(2-氨乙基)苯磺酰氟鹽酸鹽 |
別 名 | AEBSF; AEBSF, Hydrochloride; AEBSF HCl; 4-(2-氨乙基)苯磺酰氟鹽酸鹽; 苯磺酰氟鹽酸鹽; 苯磺酰氟鹽; 苯磺酰氟; AEBSF絲氨酸抑制劑; AEBSF絲氨酸蛋白酶抑制劑; |
克 隆 號 | |
CAS | 30827-99-7 |
理論分子量 | kDa |
檢測分子量 | |
保存條件 | Store at -20℃ for 3 year(Powder); In DMSO or others solvent store at 2-4℃ for two weeks, at -20℃ for six months. |
注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
產(chǎn)品介紹 |
基本信息: CAS:30827-99-7 分子式:C8H10FNO2S·HCl 分子量:239.69 MDL:MFCD00132962 級別:>97% by HPLC 溶解性:溶于水。H2O:可溶性50mg/mL(如果在pH值低于7的條件下冷藏,則可穩(wěn)定長達6個月。如果要求pH值大于7,應在使用前進行pH值調(diào)整。) 性狀:白色粉末 敏感性:易吸潮 作用靶點:Thrombin; Influenza Virus; 作用通路:Metabolic Enzyme/Protease; Anti-infection; 用途:蛋白酶抑制劑,是一種水溶性的乳糜蛋白酶、激肽釋放酶、纖溶酶、凝血酶、胰蛋白酶和相關溶栓酶的特異性不可逆抑制劑。PMSF和DFP的無毒替代品。AEBSF 在PH7的水溶液中4℃可保持穩(wěn)定1-2個月,但在低PH條件下更穩(wěn)定。推薦使用濃度為:0.1-1.0 mM。AEBSF 已用于細胞培養(yǎng),使用濃度可達 0.25mM。 體外研究: AEBSF inhibits the constitutive production of Aβ by directly inhibiting β-secretase in five different human cell lines, both neural and nonneural. AEBSF, as a serine protease inhibitor, inhibits the lysis of leukemic cells by human macrophages without inhibiting macrophage secretion of TNF-α and IL-1β. AEBSF also disturbs the growth of blastocysts on endometrial cells and inhibit the adhesion of HeLa cells on HUVECs by altering the protein secretion pattern. 體內(nèi)研究: AEBSF (76.8 mg/kg daily i.p.) results in prolongation of the survival of mice that have been given a lethal T. gondii infection. AEBSF also reduces airway response and underlying inflammation in cockroach allergen-induced murine model. |
1、抗體溶解方法 | |
2、抗體修復方式 | |
3、常用試劑的配制 | |
4、免疫組化操作步驟 | |
5、免疫組化問題解答 | |
6、Western Blotting 操作步驟 | |
7、Western Blotting 問題解答 | |
8、關于肽鏈的設計 | |
9、多肽的溶解與保存 | |
10、酶標抗體效價測定程序 | |