| 產(chǎn)品編號 | bs-22238R |
| 英文名稱 | smooth muscle Myosin heavy chain 11 Rabbit pAb |
| 中文名稱 | 平滑肌肌球蛋白重鏈抗體 |
| 別 名 | SMMHC; Myosin 11; Myosin-11; Myosin, smooth muscle, heavy chain; Myosin Smooth Muscle; AAT 4; AAT4; DKFZp686D10126; DKFZp686D19237; FAA 4; FAA4; FLJ35232; KIAA0866; MGC126726; MGC32963; MYH 11; MYH11 protein; Myosin heavy chain 11 smooth muscle; Myosin heavy chain smooth muscle isoform; Myosin heavy polypeptide 11 smooth muscle; Myosin11; SMHC; Smooth muscle myosin heavy chain isoform SM1; MYH11_HUMAN. |
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Specific References (1) | bs-22238R has been referenced in 1 publications.
[IF=10.171] Wan Zhou. et al. Retinol binding protein 4 promotes the phenotypic transformation of vascular smooth muscle cells under high glucose condition via modulating RhoA/ROCK1 pathway. TRANSL RES. 2023 Mar;: WB ; Rat.
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| 研究領(lǐng)域 | 腫瘤 心血管 免疫學(xué) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應(yīng) | Mouse (predicted: Human,Rat) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 227 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human smooth muscle Myosin heavy chain 11: 1910-1972/1972 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The protein encoded by this gene is a smooth muscle myosin belonging to the myosin heavy chain family. The gene product is a subunit of a hexameric protein that consists of two heavy chain subunits and two pairs of non-identical light chain subunits. It functions as a major contractile protein, converting chemical energy into mechanical energy through the hydrolysis of ATP. The gene encoding a human ortholog of rat NUDE1 is transcribed from the reverse strand of this gene, and its 3' end overlaps with that of the latter. The pericentric inversion of chromosome 16 [inv(16)(p13q22)] produces a chimeric transcript that encodes a protein consisting of the first 165 residues from the N terminus of core-binding factor beta in a fusion with the C-terminal portion of the smooth muscle myosin heavy chain. This chromosomal rearrangement is associated with acute myeloid leukemia of the M4Eo subtype. Alternative splicing generates isoforms that are differentially expressed, with ratios changing during muscle cell maturation. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008] Function: Muscle contraction. Subunit: Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2). Subcellular Location: Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Thick filaments of the myofibrils. Tissue Specificity: Smooth muscle; expressed in the umbilical artery, bladder, esophagus and trachea. DISEASE: Note=A chromosomal aberration involving MYH11 is found in acute myeloid leukemia of M4EO subtype. Pericentric inversion inv(16)(p13;q22). The inversion produces a fusion protein consisting of the 165 N-terminal residues of CBF-beta (PEPB2) and the tail region of MYH11. Familial aortic aneurysm thoracic 4 (AAT4) [MIM:132900]: A disease characterized by permanent dilation of the thoracic aorta usually due to degenerative changes in the aortic wall. It is primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance. Note=The disease is caused by mutations affecting the gene represented in this entry. Similarity: Contains 1 IQ domain. Contains 1 myosin head-like domain. SWISS: P35749 Gene ID: 4629 Database links: Entrez Gene: 4629 Human Entrez Gene: 17880 Mouse Omim: 160745 Human SwissProt: P35749 Human SwissProt: O08638 Mouse SwissProt: P35748 Rabbit 平滑肌肌球蛋白重鏈是分子量為200kDa的多肽，是六聚肌球蛋白的結(jié)構(gòu)成分。主要用于標記血管和內(nèi)臟平滑肌細胞以及肌上皮細胞，被認為是平滑肌較為特異和可靠的標志，有助于間葉腫瘤的診斷和分類，也可用于乳腺肌上皮細胞的檢測，有助于原位癌和浸潤癌的區(qū)別鑒定。 |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (smooth muscle Myosin heavy chain 11) Polyclonal Antibody, Unconjugated (bs-22238R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
