| 產(chǎn)品編號 | bsm-51210M |
| 英文名稱 | CYP1A1 Mouse mAb |
| 中文名稱 | 細(xì)胞色素P450 1A1單克隆抗體 |
| 別 名 | Cytochrome p450 1A1; AHH; AHRR; CP11; CYP1; P1-450; P450-C; P450DX; AHH; AHRR; Aryl hydrocarbon hydroxylase; CYP 1; CYP1A1; CYPIA1; Cytochrome P1-450; Flavoprotein-linked monooxygenase; Microsomal monooxygenase; P1 450; P450 C; P450 form 6; P450 P1; Xenobiotic monooxygenase. |
| 研究領(lǐng)域 | 細(xì)胞生物 免疫學(xué) 合成與降解 |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號 | 10G3 |
| 交叉反應(yīng) | Human (predicted: Mouse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 58 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞漿 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CYP1A1: 377-405/512 |
| 亞 型 | IgG1 |
| 純化方法 | affinity purified by Protein G |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The Cytochrome P450 (P450) superfamily of enzymes is one of three enzyme systems which metabolize the fatty acid arachadonic acid (AA) to regulators of vascular tone. P450 enzymes are monooxygenase enzymes which require several cofactors such as nicotinamide adenine dinucleotide phosphate (NADPH) and P450 reductase. There are over 200 known genes which encode P450s. Epoxygenases are those P450s which metabolize AA to epoxyeicosatrienoic acid (EETs) and omega hydroxylases are those P450s which produce 19- and 20-hydroxyeicosatetraenoic acids (19- and 20-HETE). As well as fatty acid metabolism, P450s also metabolize many drugs and toxins. Cytochrome P450 3A4 is abundantly expressed in liver and small intestine and is inducible by barbiturates, glucocorticoids and rifampicin. Function: Mediates dioxin toxicity and is involved in regulation of cell growth and differentiation. Represses the transcription activity of AHR by competing with this transcription factor for heterodimer formation with the ARNT and subsequently binding to the xenobiotic response element (XRE) sequence present in the promoter regulatory region of variety of genes. Represses CYP1A1 by binding the XRE sequence and recruiting ANKRA2, HDAC4 and/or HDAC5. Autoregulates its expression by associating with its own XRE site. Subcellular Location: Cytochrome P450 1A1: Cytoplasm. Cytochrome P450MT2A: Endoplasmic reticulum membrane. Mitochondrion membrane. Microsome membrane. Cytochrome P450MT2B: Endoplasmic reticulum. Mitochondrion. Tissue Specificity: Highly expressed in testis, lung, ovary, spleen and pancreas. Highly expressed in mononuclear cells (MNCs) from umbilical cord blood. Isoform 3 is highly expressed in lung, kidney, spleen and thymus. Down-regulated malignant tissue from different anatomical origins, including colon, breast, lung, stomach, cervix, and ovary. Similarity: Belongs to the cytochrome P450 family. SWISS: A9YTQ3 Gene ID: 57491 Database links: Entrez Gene: 57491 Human Entrez Gene: 11624 Mouse Omim: 606517 Human SwissProt: A9YTQ3 Human SwissProt: Q3U1U7 Mouse Unigene: 50823 Human Unigene: 290446 Mouse Unigene: 145524 Rat |
| 產(chǎn)品圖片 |
Sample:
A549(Human) Cell Lysate at 30 ug
SiHa(Human) Cell Lysate at 30 ug
Primary: Anti-CYP1A1 (bsm-51210M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 58 kD
Observed band size: 58 kD
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