| 產(chǎn)品編號(hào) | bs-20413R |
| 英文名稱 | Rabbit Anti-TGF-beta 3 antibody |
| 中文名稱 | 轉(zhuǎn)移生長因子β3抗體（TGFβ3） |
| 別 名 | Transforming growth factor beta-3 proprotein; TGFB3; ARVD; FLJ16571; MGC105479; MGC118722; TGF beta 3; TGF beta3; TGF-beta-3; TGF-beta 3; TGFB 3; Transforming growth factor beta-3; TGFB3_HUMAN. |
| 研究領(lǐng)域 | 信號(hào)轉(zhuǎn)導(dǎo) 干細(xì)胞 生長因子和激素 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse (predicted: Rat) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,Flow-Cyt=1ug/Test,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 細(xì)胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human TGF-beta 3: 321-412/412 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | Preservative: 0.02% Proclin300, Constituents: 1% BSA, 0.01M PBS, pH7.4. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The TGFBeta superfamily is composed of numerous growth and differentiation factors, including transforming growth factors Beta(TGFBeta) 1,2 and 3; placental transforming growth factor (PTGFBeta);growth /differentiation factors (GDFs); mullerian inhibiting substance (MIS);bone morphogenic proteins (BMPs);glial cell line-derived neurotrophic factor (GDNF);inhibins or activins (Alpha,Beta-A, Beta-B and Beta-C),Lefty and Nodal. Members of the TGF superfamily are involved in embryonic development and adult tissue homeostasis. Function: Involved in embryogenesis and cell differentiation. Subunit: Homodimer; disulfide-linked. Interacts with ASPN. Subcellular Location: Secreted. DISEASE: Defects in TGFB3 are a cause of familial arrhythmogenic right ventricular dysplasia type 1 (ARVD1) [MIM:107970]; also known as arrhythmogenic right ventricular cardiomyopathy 1 (ARVC1). ARVD is an autosomal dominant disease characterized by partial degeneration of the myocardium of the right ventricle, electrical instability, and sudden death. It is clinically defined by electrocardiographic and angiographic criteria; pathologic findings, replacement of ventricular myocardium with fatty and fibrous elements, preferentially involve the right ventricular free wall. Similarity: Belongs to the TGF-beta family. SWISS: P10600 Gene ID: 7043 Database links: Entrez Gene: 7043 Human Entrez Gene: 21809 Mouse Omim: 190230 Human SwissProt: P10600 Human SwissProt: P17125 Mouse Unigene: 592317 Human Unigene: 3992 Mouse Unigene: 7018 Rat 生長因子和激素（ Growth Factor and Hormones） TGF是一種多效生長因子,對(duì)上皮細(xì)胞增值有潛在抑制作用,可抑制腫瘤生長，用于許多惡性腫瘤如:胃癌、肺癌、膀胱癌、腎癌、前列腺癌、結(jié)腸癌等多種惡性腫瘤的研究。分子量：47kDa。此抗體是TGFβ3 特異性抗體，與TGF-β1和TGF-β2沒有交叉反應(yīng)。 |
| 產(chǎn)品圖片 |
Sample:Ovary (Mouse)Lysate at 40 ug
Primary: Anti-TGF-beta 3(bs-20413R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 30'47kD
Observed band size: 47kD
Blank control（black line）:SH-SY5Y.
Primary Antibody (green line): Rabbit Anti-TGF-beta 3 antibody (bs-20413R)
Dilution:1ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 0.5ug/Test.
Negative control（white blue line）: PBS
Isotype control（orange line）: Normal Rabbit IgG
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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