| 產(chǎn)品編號 | bs-19229R |
| 英文名稱 | Rabbit Anti-NFYA antibody |
| 中文名稱 | 核轉(zhuǎn)錄因子YA抗體 |
| 別 名 | CAAT box DNA binding protein subunit A; CAAT box DNA-binding protein subunit A; CBF A; CBF B; CBFA; CBFB; CCAAT binding transcription factor subunit B; FLJ11236; HAP 2; HAP2; HAP2 CCAAT binding protein; NF YA; NF-YA; NFY protein chain A; NFYA; NFYA_HUMAN; NUCLEAR FACTOR BINDING TO Y BOX OF HLA GENES; Nuclear transcription factor Y alpha; Nuclear transcription factor Y; Nuclear transcription factor Y subunit A; Nuclear transcription factor Y subunit alpha; Sez10; Transcription factor NF Y A subunit. |
| 研究領域 | 細胞生物 轉(zhuǎn)錄調(diào)節(jié)因子 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | (predicted: Human,Mouse,Rat,Rabbit,Pig,Sheep,Cow,Chicken,Dog,Horse) |
| 產(chǎn)品應用 | IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 37kDa |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NFYA: 251-347/347 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds to CCAAT motifs in the promoter regions in a variety of genes. Subunit A associates with a tight dimer composed of the B and C subunits, resulting in a trimer that binds to DNA with high specificity and affinity. The sequence specific interactions of the complex are made by the A subunit, suggesting a role as the regulatory subunit. In addition, there is evidence of post-transcriptional regulation in this gene product, either by protein degradation or control of translation. Further regulation is represented by alternative splicing in the glutamine-rich activation domain, with clear tissue-specific preferences for the two isoforms. [provided by RefSeq, Jul 2008] Function: Stimulates the transcription of various genes by recognizing and binding to a CCAAT motif in promoters, for example in type 1 collagen, albumin and beta-actin genes. Subcellular Location: Nucleus. Similarity: Belongs to the NFYA/HAP2 subunit family. SWISS: P23511 Gene ID: 4800 Database links: Entrez Gene: 4800 Human Entrez Gene: 18044 Mouse Omim: 189903 Human SwissProt: P23511 Human SwissProt: P23708 Mouse Unigene: 10441 Human Unigene: 4929 Mouse Unigene: 10747 Rat |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
