| 產(chǎn)品編號 | bs-17387R |
| 英文名稱 | HRC Rabbit pAb |
| 中文名稱 | HRC蛋白抗體 |
| 別 名 | HCP; HRC; HRC histidine rich calcium binding protein; MGC133236; Sarcoplasmic reticulum histidine-rich calcium-binding protein; SRCH_HUMAN. |
| 研究領(lǐng)域 | 細(xì)胞生物 免疫學(xué) 結(jié)合蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應(yīng) | Human |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 77 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞外基質(zhì) |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human HRC: 21-120/699 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
HRC is a 699 amino acid that binds low-density lipoprotein with high affinity. As a high capacity calcium binding protein, HRC regulates the sequestration and release of calcium in the lumen of the junctional sarcoplasmic reticulum (SR) of cardiac, skeletal and smooth muscle. This mechanism may involve direct interaction between HRC and the cytoplasmic domain of Triadin, an integral membrane protein of the SR. The gene encoding HRC maps to human chromosome 19q13.33 and mouse chromosome 7 B4 Function: May play a role in the regulation of calcium sequestration or release in the SR of skeletal and cardiac muscle. Subcellular Location: Sarcoplasmic reticulum lumen. Similarity: To rabbit HRC. SWISS: P23327 Gene ID: 3270 Database links: Entrez Gene: 3270 Human Omim: 142705 Human SwissProt: P23327 Human Unigene: 436885 Human |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (human heart tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HRC) Polyclonal Antibody, Unconjugated (bs-17387R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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