| 產(chǎn)品編號 | bs-11250R |
| 英文名稱 | EEA1 Rabbit pAb |
| 中文名稱 | 早期內(nèi)吞體相關(guān)蛋白1抗體 |
| 別 名 | Early endosome antigen 1; Early endosome antigen 1, 162kD; Early endosome associated protein; EEA 1; EEA1; EEA1_HUMAN; Endosome associated protein p162; Endosome-associated protein p162; MST105; MSTP105; ZFYVE2; Zinc finger FYVE domain containing protein 2; Zinc finger FYVE domain-containing protein 2. |
| 研究領(lǐng)域 | 細胞生物 神經(jīng)生物學(xué) 信號轉(zhuǎn)導(dǎo) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Hamster (predicted: Mouse,Rat,Cow,Dog) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1ug/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 162 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human EEA1: 1251-1350/1411 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Early endosomes are cytoplasmic compartments that function in receiving and sorting endocytosed proteins for vesicular transport. EEA1 (early endosome antigen 1) is a peripheral membrane protein that co-localizes with the transferrin receptor and Rab5 on early endosomes. EEA1 contains a calmodulin-binding IQ motif and cysteine rich finger motif necessary for its specific localization to the early endosomes. EEA1 has sequence homology to several yeast proteins that have been implicated in membrane trafficking, including Vps27, Fab1 and Vac1. Evidence suggests a possible role for EEA1 in mediating the regulatory effects of 3'-phosphoinositides on membrane trafficking. Function: Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking. Subunit: Homodimer. Binds STX6. Binds RAB5A, RAB5B, RAB5C and RAB22A that have been activated by GTP-binding. Interacts with ERBB2. Subcellular Location: Cytoplasm. Early endosome membrane. Similarity: Contains 1 C2H2-type zinc finger. Contains 1 FYVE-type zinc finger. SWISS: Q15075 Gene ID: 8411 Database links: Entrez Gene: 8411 Human Entrez Gene: 216238 Mouse Omim: 605070 Human SwissProt: Q15075 Human SwissProt: Q8BL66 Mouse Unigene: 567367 Human Unigene: 210035 Mouse Unigene: 23513 Rat |
| 產(chǎn)品圖片 |
Tissue/cell: human stomach tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goa
Blank control（black line）:Hela.
Primary Antibody (green line): Rabbit Anti-EEA1 antibody (bs-11250R)
Dilution:1ug/Test;
Secondary Antibody（white blue line）: Goat anti-rabbit IgG-AF488
Dilution: 0.5ug/Test.
Isotype control（orange line）: Normal Rabbit
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
