| 產(chǎn)品編號 | bs-9901R |
| 英文名稱 | PAFAHG Rabbit pAb |
| 中文名稱 | 血小板衍化生長因子γ抗體 |
| 別 名 | PA1B3_HUMAN; PAF acetylhydrolase 29 kDa subunit; PAF AH 29 kDa subunit; PAF AH subunit gamma; PAF-AH 29 kDa subunit; PAF-AH subunit gamma; PAFAH subunit gamma; Pafah1b3; PAFAHG; Platelet activating factor acetylhydrolase IB subunit gamma; Platelet activat |
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Specific References (1) | bs-9901R has been referenced in 1 publications.
[IF=0] Vandenberghe L T M et al. Platelet-activating factor acetylhydrolase 1B3 (PAFAH1B3) is required for the formation of the meiotic spindle during invitro oocyte maturation.Reproduction, Fertility and Development.2018. WB&ICF ; Bovine, Murine&Human.
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| 研究領(lǐng)域 | 腫瘤 心血管 神經(jīng)生物學(xué) 信號轉(zhuǎn)導(dǎo) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Mouse,Rat (predicted: Human,Rabbit,Pig,Sheep,Cow,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 26 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PAFAH1B3/PAFAHG: 1-100/231 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The platelet activating factor (PAF) acetylhydrolases catalyze hydrolysis of the sn-2 ester bond of PAF and related pro-inflammatory phospholipids and thus attenuate their bioactivity. The family of PAF acetylhydrolases includes one secreted plasma isozyme and two intracellular isozymes. The intracellular isozymes are distinguished by differences in their primary sequence, tissue localization, subunit composition and substrate preferences. The most thoroughly characterized intracellular isoform, PAFAH1B, is a heterotrimeric protein expressed in brain tissue and plays and important role in brain development and function. PAFAH1B is comprised of a regulatory subunit (LIS1) and two homologous (63% identity) catalytic subunits (PAFAH1B2 and PAFAH1B3), which harbor all the activity of the enzyme. The PAFAH1B2 and PAFAH1B3 subunits readily associate with very high affinity to form heterodimers, and this dimerization is essential for both stability and catalytic activity. PAFAH1B3 is also commonly known as PAFAH1B 29kDa subunit, PAFAH1B subunit or PAFAH1B subunit α1. Function: Inactivates paf by removing the acetyl group at the sn-2position. This is a catalytic subunit. Plays an important roleduring the development of brain. Subunit: Cytosolic PAF-AH IB is formed of three subunits of 45 kDa(alpha), 30 kDa (beta) and 29 kDa (gamma). The catalytic activityof the enzyme resides in the beta and gamma subunits, whereas thealpha subunit has regulatory activity. Trimer formation is notessential for the catalytic activity. Subcellular Location: Cytoplasm. Tissue Specificity: In the adult, expressed in brain, skeletal muscle, kidney, thymus, spleen, colon, testis, ovary and peripheral blood leukocytes. In the fetus, highest expression occurs in brain. Similarity: Belongs to the 'GDSL' lipolytic enzyme family.Platelet-activating factor acetylhydrolase IB beta/gamma subunitssubfamily. SWISS: Q15102 Gene ID: 5050 Database links: Entrez Gene: 5050 Human Entrez Gene: 18476 Mouse Omim: 603074 Human SwissProt: Q15102 Human SwissProt: Q61205 Mouse Unigene: 466831 Human Unigene: 597 Mouse Unigene: 17971 Rat |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PAFAHG) Polyclonal Antibody, Unconjugated (bs-9901R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PAFAHG) Polyclonal Antibody, Unconjugated (bs-9901R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
