| 產(chǎn)品編號(hào) | bs-8163R |
| 英文名稱(chēng) | TCAB1 Rabbit pAb |
| 中文名稱(chēng) | 端粒酶卡哈爾體蛋白抗體 |
| 別 名 | FLJ10385; TCAB1; telomerase Cajal Body protein 1; WD repeat containing protein 79; WRAP53; WDR79/TCAB1; WAP53_HUMAN. |
| 研究領(lǐng)域 | 腫瘤 細(xì)胞生物 免疫學(xué) |
| 抗體來(lái)源 | Rabbit |
| 克隆類(lèi)型 | Polyclonal |
| 克 隆 號(hào) | |
| 交叉反應(yīng) | Human,Mouse,Rat (predicted: Rabbit,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 60 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞核 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human WDR79/TCAB1: 285-390/548 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
WDR79 contains six WD (tryptophan-aspartate) repeat domains found in a number of proteins that function as adaptor molecules in signal transduction and cytoskeletal organization. The WD repeat is defined by four or more repeating units of a conserved core of approximately 40 amino acids ending with tryptophan-aspartic acid (WD). WD repeats may serve as sites of protein-protein interaction for adaptor proteins and facilitate multiprotein complex formation. The function of the WDR79 protein has not been characterized, however significant and consistent single nucleotide polymorphisms in the WDR79 gene have been found to be associated with ER negative breast cancer. Function: Essential component of the telomerase holoenzyme complex, a ribonucleoprotein complex essential for the replication of chromosome termini that elongates telomeres in most eukaryotes. In the telomerase holoenzyme complex, it controls telomerase localization to Cajal body. Required for delivery of TERC to telomeres during S phase and for telomerase activity. Binds small Cajal body RNAs (scaRNAs). The mRNA encoding this protein plays a critical role in the regulation of p53 expression at the post-transcriptional level; it is involved both in maintaining basal p53 mRNA levels and in p53 induction upon DNA damage. Subunit: Component of the telomerase holoenzyme complex at least composed of TERT, DKC1, WRAP53/TCAB1, NOP10, NHP2, GAR1, TEP1, EST1A, POT1 and a telomerase RNA template component (TERC). Subcellular Location: Nucleus, Cajal body. Cytoplasm. Tissue Specificity: Expressed in all tissues and cell lines examined. Post-translational modifications: Phosphorylated upon DNA damage, probably by ATM or ATR. DISEASE: Defects in WRAP53 are the cause of dyskeratosis congenital autosomal recessive type 3 (DKCB3) [MIM:613988]. A rare multisystem disorder caused by defective telomere maintenance. It is characterized by progressive bone marrow failure, and the clinical triad of reticulated skin hyperpigmentation, nail dystrophy, and mucosal leukoplakia. Common but variable features include premature graying, aplastic anemia, low platelets, osteoporosis, pulmonary fibrosis, and liver fibrosis among others. Early mortality is often associated with bone marrow failure, infections, fatal pulmonary complications, or malignancy. Similarity: Contains 6 WD repeats SWISS: Q9BUR4 Gene ID: 55135 Database links: Entrez Gene: 55135 Human Omim: 612661 Human SwissProt: Q9BUR4 Human Unigene: 437460 Human TCAB1是端粒酶的一個(gè)真正組成部分。但它對(duì)酶的活性來(lái)說(shuō)并不是必需的，它只是給稱(chēng)為卡哈爾體（Cajalbodies）的細(xì)胞核中的處理和保持區(qū)域補(bǔ)充端粒酶復(fù)合物?？ü栿w將對(duì)各種使用RNA小分子來(lái)引領(lǐng)其活性的蛋白進(jìn)行修飾，譬如，端粒酶使用RNA分子作為嵌在染色體末端的DNA鏈的模板。在適當(dāng)?shù)臅r(shí)候，TCAB1將端粒酶復(fù)合物運(yùn)送到新復(fù)制染色體的等待端。 研究人員認(rèn)為，TCAB1蛋白可能是一種負(fù)責(zé)將各種分子運(yùn)往其目的地的普通生物運(yùn)輸器。 |
| 產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with TCAB1 polyclonal antibody, unconjugated (bs-8163R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded (rat ovary tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TCAB1) Polyclonal Antibody, Unconjugated (bs-8163R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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