| 產(chǎn)品編號(hào) | bs-7723R |
| 英文名稱 | REC8 Rabbit pAb |
| 中文名稱 | 減數(shù)分裂重組蛋白家族REC8抗體 |
| 別 名 | Cohesin Rec8p; Meiotic recombination protein REC8 homolog; Meiotic recombination protein REC8-like 1; rec8; REC8 homolog(yeast); REC8_HUMAN; REC8L; Rec8L1; Rec8p. |
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Specific References (2) | bs-7723R has been referenced in 2 publications.
[IF=6.831] Shangren Wang. et al. Long noncoding RNAs regulated spermatogenesis in varicocele-induced spermatogenic dysfunction. 2022 Mar 17 WB ; Rat.
[IF=1.635] Shimoi G et al.Destabilization of spindle assembly checkpoint causes aneuploidy during meiosis II in murine post-ovulatory aged oocytes.(2018)J Reprod Dev. WB ; Mouse.
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| 研究領(lǐng)域 | 細(xì)胞生物 細(xì)胞周期蛋白 細(xì)胞分化 表觀遺傳學(xué) |
| 抗體來(lái)源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse,Rat (predicted: Rabbit,Pig,Dog,GuineaPig,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,Flow-Cyt=1ug/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 60 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human REC8: 22-120/547 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Required during meiosis for separation of sister chromatids and homologous chromosomes. Proteolytic cleavage of REC8 on chromosome arms by separin during anaphase I allows for homologous chromosome separation in meiosis I and cleavage of REC8 on centromeres during anaphase II allows for sister chromatid separation in meiosis II. Function: Required during meiosis for separation of sister chromatids and homologous chromosomes. Proteolytic cleavage of REC8 on chromosome arms by separin during anaphase I allows for homologous chromosome separation in meiosis I and cleavage of REC8 on centromeres during anaphase II allows for sister chromatid separation in meiosis II (By similarity). Subunit: Interacts (phosphorylated and unphosphorylated form) with SMC3. Interacts with SYCP3. Interacts (phosphorylated and unphosphorylated form) with SMC1B. Does not interact with SMC1A. Interacts with RAD51. Subcellular Location: Nucleus. Chromosome. Tissue Specificity: Expressed in testis and thymus. Similarity: Belongs to the rad21 family. SWISS: O95072 Gene ID: 9985 Database links: Entrez Gene: 9985 Human Entrez Gene: 56739 Mouse Omim: 608193 Human SwissProt: O95072 Human SwissProt: Q8C5S7 Mouse Unigene: 419259 Human Unigene: 23149 Mouse Unigene: 108612 Rat
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| 產(chǎn)品圖片 |
Sample: HT29 Cell (Human) Lysate at 40 ug
Primary: Anti-REC8 (bs-7723R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60 kD
Observed band size: 65 kD
Sample:
Thymus (Mouse) Lysate at 40 ug
Primary: Anti-REC8? (bs-7723R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60 kD
Observed band size: 60 kD
Blank control (Black line):Molt4 (Black).
Primary Antibody (green line): Rabbit Anti-REC8 antibody (bs-7723R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-
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