| 產(chǎn)品編號(hào) | bs-7721R |
| 英文名稱(chēng) | CAPG2 Rabbit pAb |
| 中文名稱(chēng) | 染色體相關(guān)蛋白2抗體 |
| 別 名 | NCAPG2; Chromosome associated protein G2; LUZP5; CAP G2; Leucine zipper protein 5; Non SMC condensin II complex subunit G2; Condensin 2 complex subunit G2; NCAPG 2; Non SMC condensin II complex, subunit G2; CNDG2_HUMAN. |
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Specific References (1) | bs-7721R has been referenced in 1 publications.
[IF=8.44] Wang Wei. et al. Circular RNA circ0001955 promotes cervical cancer tumorigenesis and metastasis via the miR-188-3p/NCAPG2 axis. J TRANSL MED. 2023 Dec;21(1):1-18 IHC,WB ; Human,Mouse.
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| 研究領(lǐng)域 | 細(xì)胞生物 細(xì)胞周期蛋白 細(xì)胞分化 表觀遺傳學(xué) |
| 抗體來(lái)源 | Rabbit |
| 克隆類(lèi)型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse,Rat |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=2ug/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 131 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CAPG2/NCAPG2: 451-550/1143 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
NCAPG2 is 1 of 3 non-SMC subunits that define condensin II. Condensin complexes I and II play essential roles in mitotic chromosome assembly and segregation. Both condensins contain 2 invariant structural maintenance of chromosome (SMC) subunits, SMC2 and SMC4, but they contain different sets of non-SMC subunits. NCAPG2 is 1 of 3 non-SMC subunits that defines condensin II. Function: Regulatory subunit of the condensin-2 complex, a complex which establishes mitotic chromosome architecture and is involved in physical rigidity of the chromatid axis. Subunit: Component of the condensin-2 complex, which contains the SMC2 and SMC4 heterodimer, and 3 non SMC subunits that probably regulate the complex: NCAPH2, NCAPD3 and NCAPG2. Subcellular Location: Nucleus. Similarity: Contains 1 HEAT repeat. SWISS: Q86XI2 Gene ID: 54892 Database links: Entrez Gene: 54892 Human Omim: 608532 Human SwissProt: Q86XI2 Human |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Mouse Thymus tissue lysates
Lane 2: Rat Testis tissue lysates
Lane 3: Human A431 cell lysates
Lane 4: Human MCF-7 cell lysates
Lane 5: Human HeLa cell lysates
Lane 6: Human U251 cell lysates
Lane 7: Human Jurkat cell lysates
Primar
Sample:
293T(Human) Cell Lysate at 30 ug
Primary: Anti-CAPG2 (bs-7721R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 131 kD
Observed band size: 131 kD
Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; I
Blank control:Mouse spleen.
Primary Antibody (green line): Rabbit Anti-CAPG2 antibody (bs-7721R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Pr
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