| 產(chǎn)品編號 | bs-1635R |
| 英文名稱 | B3GAT1 Rabbit pAb |
| 中文名稱 | 髓鞘相關(guān)糖蛋白（CD57）抗體 |
| 別 名 | 3-glucuronyltransferase 1; 3-glucuronyltransferase; B3GA1_HUMAN; beta 1 3 glucuronyltransferase 1; beta-1; CD 57; CD57; CD57 antigen; Galactosylgalactosylxylosylprotein 3 beta glucuronosyltransferase 1; Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1; GlcAT P; GlcAT-P; GLCATP; GlcUAT P; GlcUAT-P; GlcUATP; Glucuronosyltransferase P; HNK 1; HNK1; LEU7; LEU7 antigen; NK 1; NK1; UDP GlcUA glycoprotein beta 1 3 glucuronyltransferase; UDP-GlcUA:glycoprotein beta-1. |
 |
Specific References (2) | bs-1635R has been referenced in 2 publications.
[IF=5.29] Kaneko, Yuji, et al. "Kainic Acid-Induced Golgi Complex Fragmentation/Dispersal Shifts the Proteolysis of Reelin in Primary Rat Neuronal Cells: An In Vitro Model of Early Stage Epilepsy." Molecular Neurobiology (2015): 1-10. WB ; Rat.
[IF=3.6] Salisbury, Elizabeth A., et al. "Transient Brown Adipocyte-Like Cells Derive from Peripheral Nerve Progenitors in Response to Bone Morphogenetic Protein 2." Stem cells translational medicine 1.12 (2012): 874-885.
|
| 研究領(lǐng)域 | 細(xì)胞生物 免疫學(xué) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human (predicted: Mouse,Rat,Chicken) |
| 產(chǎn)品應(yīng)用 | Flow-Cyt=2ug/Test,ICC/IF=1:100
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 38 kDa |
| 檢測分子量 | 45 |
| 細(xì)胞定位 | 細(xì)胞漿 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human B3GAT1: 21-120/334 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The protein encoded by this gene is a member of the glucuronyltransferase gene family. These enzymes exhibit strict acceptor specificity, recognizing nonreducing terminal sugars and their anomeric linkages. This gene product functions as the key enzyme in a glucuronyl transfer reaction during the biosynthesis of the carbohydrate epitope HNK-1 (human natural killer-1, also known as CD57 and LEU7). Alternate transcriptional splice variants have been characterized. [provided by RefSeq, Jul 2008] Function: Involved in the biosynthesis of L2/HNK-1 carbohydrate epitope on glycoproteins. Can also play a role in glycosaminoglycan biosynthesis. Substrates include asialo-orosomucoid (ASOR), asialo-fetuin, and asialo-neural cell adhesion molecule. Requires sphingomyelin for activity: stearoyl-sphingomyelin was the most effective, followed by palmitoyl-sphingomyelin and lignoceroyl-sphingomyelin. Activity was demonstrated only for sphingomyelin with a saturated fatty acid and not for that with an unsaturated fatty acid, regardless of the length of the acyl group. Subunit: Homodimer (Potential). Subcellular Location: Golgi apparatus membrane; Single-pass type II membrane protein. Tissue Specificity: Mainly expressed in the brain. Similarity: Belongs to the glycosyltransferase 43 family. SWISS: Q9P2W7 Gene ID: 27087 Database links: Entrez Gene: 27087 Human Omim: 151290 Human SwissProt: Q9P2W7 Human Unigene: 381050 Human CD57是一種分子量為110KD的糖蛋白,它是自然殺傷細(xì)胞(NK)和殺傷細(xì)胞的特異性表面抗原。CD57表達(dá)于15-20%的外周血單核細(xì)胞，60%的NK活化細(xì)胞以及T細(xì)胞亞群。該抗體可以標(biāo)記NK細(xì)胞，也可識別非淋巴組織的一些細(xì)胞?？捎糜贜K細(xì)胞介導(dǎo)的細(xì)胞毒、NK細(xì)胞及T細(xì)胞亞群的功能等方面的研究，也可標(biāo)記神經(jīng)內(nèi)分泌細(xì)胞及其腫瘤。 |
| 產(chǎn)品圖片 |
SH-SY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (B3GAT1) polyclonal Antibody, Unconjugated (bs-1635R) 1:100, 90 minutes at 37°C;
Blank control: U2OS.
Primary Antibody (green line): Rabbit Anti-CD57 antibody (bs-1635R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
T
|
