| 產(chǎn)品編號 | bs-1461R |
| 英文名稱 | Rabbit Anti-VDAC antibody |
| 中文名稱 | 等電壓依賴性陰離子通道（內(nèi)參）抗體 |
| 別 名 | voltage-dependent anion channel; hVDAC1; MGC111064; Outer mitochondrial membrane protein porin 1; Plasmalemmal porin; Porin 31 HL; Porin 31HL; Porin 31HM; VDAC 1; VDAC; VDAC1; Voltage dependent anion channel 1; Voltage dependent anion selective channel protein 1; VDAC1_HUMAN. |
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Specific References (7) | bs-1461R has been referenced in 7 publications.
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| 產(chǎn)品類型 | 內(nèi)參抗體 |
| 研究領(lǐng)域 | 細胞生物 免疫學 細胞凋亡 通道蛋白 線粒體 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Rat (predicted: Mouse,Rabbit,Pig,Sheep,Cow,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 32kDa |
| 細胞定位 | 細胞漿 細胞膜 線粒體 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human VDAC: 85-190/283 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Voltage dependent anion selective channel protein 1 (VDAC/Porin) belongs to the eukaryotic mitochondrial porin family and forms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel allows diffusion of small hydrophilic molecules; it adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation selective. VDAC/Porin expression is observed in the heart,liver and skeletal muscle. Function: Involved in the endosomal multivesicular bodies (MVB) pathway. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. Thought to be a cofactor of VPS4A/B, which catalyzes disassembles membrane-associated ESCRT-III assemblies. Involved in the sorting and down-regulation of EGFR (By similarity). Involved in HIV-1 budding. Subunit: Interacts with VPS4B. Interacts with CHMP1B. Interacts with CHMP2A; the interaction probably involves the open conformation of (polymerized) CHMP2A. May interact with CHMP3. Interacts with CHMP5; the interaction involves soluble CHMP5. Interacts with IST1. Subcellular Location: Cytoplasm. Endosome membrane; Peripheral membrane protein (Probable). Similarity: Belongs to the VTA1 family. SWISS: P21796 Gene ID: 7416 Database links: Entrez Gene: 416320 Chicken Entrez Gene: 7416 Human Entrez Gene: 22333 Mouse Entrez Gene: 334582 Zebrafish Omim: 604492 Human 電壓依賴性陰離子通道VDAC是存在于線粒體外膜上的31kDa蛋白,能在膜上形成親水性通道,調(diào)控陰離子、陽離子、ATP以及其他代謝物進出線粒體,在調(diào)節(jié)細胞代謝、維持胞內(nèi)鈣穩(wěn)態(tài),調(diào)節(jié)細胞凋亡和壞死等過程中發(fā)揮重要功能。 |
| 產(chǎn)品圖片 |
Sample:
Jurkat(Human) Cell Lysate at 30 ug
Primary: Anti-VDAC (bs-1461R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 32 kD
Observed band size: 25 kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-VDAC Polyclonal Antibody, Unconjugated(bs-1461R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
