| 產(chǎn)品編號 | bs-0813R |
| 英文名稱 | Alpha casein Rabbit pAb |
| 中文名稱 | 酪蛋白抗體 |
| 別 名 | Casein; alpha-S1-casein; CASA; CASA1_Cow; Casein alpha s1; casein, alpha; casein, alpha-S1, included; Casoxin-D; CSN1; CSN1S1; MGC149368. |
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Specific References (20) | bs-0813R has been referenced in 20 publications.
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| 研究領域 | 細胞生物 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Cow,Bovine |
| 產(chǎn)品應用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 23 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞膜 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Casein from bovine milk |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Casein is the name for a family of related phosphoproteins (αS1, αS2, β, κ). These proteins are commonly found in mammalian milk, making up 80% of the proteins in cow milk and between 20% and 45% of the proteins in human milk. Casein has a wide variety of uses, from being a major component of cheese, to use as a food additive, to a binder for safety matches. As a food source, casein supplies amino acids; carbohydrates; and two inorganic elements, calcium and phosphorus. Subunit: Casein contains a fairly high number of proline residues, which do not interact. There are also no disulfide bridges. As a result, it has relatively little tertiary structure. It is relatively hydrophobic, making it poorly soluble in water. It is found in milk as a suspension of particles called "casein micelles" which show only limited resemblance with surfactant-type micellae in a sense that the hydrophilic parts reside at the surface and they are spherical. However, in sharp contrast to surfactant micelles, the interior of a casein micelle is highly hydrated. The caseins in the micelles are held together by calcium ions and hydrophobic interactions. Several models account for the special conformation of casein in the micelles. One of them proposes the micellar nucleus is formed by several submicelles, the periphery consisting of microvellosities of κ-casein. Another model suggests the nucleus is formed by casein-interlinked fibrils. Finally, the most recent model proposes a double link among the caseins for gelling to take place. All three models consider micelles as colloidal particles formed by casein aggregates wrapped up in soluble κ-casein molecules. The isoelectric point of casein is 4.6. Since milk's pH is 6.6, casein has a negative charge in milk. The purified protein is water insoluble. While it is also insoluble in neutral salt solutions, it is readily dispersible in dilute alkalis and in salt solutions such as sodium oxalate and sodium acetate. The enzyme trypsin can hydrolyze off a phosphate-containing peptone. It is used to form a type of organic adhesive. SWISS: P02662 Gene ID: 282208 Database links: Entrez Gene: 282208 Bovin Entrez Gene: 1446?Human Omim: 115450?Human SwissProt: P47710?Human Unigene: 3155?Human 酪蛋白(casein)是牛乳包括牛初乳的主要成分，通常在分離提取乳清過程中可得到大量的酪蛋白，它具有各種必需氨基酸，且組分合理，是一種優(yōu)質的動物蛋白。然而，由于人體尤其是兒童對牛乳酪蛋白的分解能力較弱，牛乳酪蛋白的應用并不廣泛。近來國外文獻報道，牛乳酪蛋白短鏈肽除了易吸收外，還具有某些重要的生物學功能，提示著酪蛋白除單純營養(yǎng)作用之外的廣闊應用前景。 |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Recombinant Bovine Alpha s1 Casein
Primary: Anti-Alpha casein (bs-0813R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 23 kDa
Observed band size: 34 kDa
25 ug total protein per lane of various lysates (see on figure) probed with Alpha casein polyclonal antibody, unconjugated (bs-0813R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
500 ng rbAlpha s1-His protein (bs-42025P) per lane probed with Alpha casein polyclonal antibody respectively, unconjugated (bs-0813R) at 1:1000 dilution and 4°C overnight incubation. Followed by corresponding conjugated secondary antibody incubation at r.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
