| 產(chǎn)品編號 | bsm-61454R |
| 英文名稱 | Tyrosinase Recombinant Rabbit mAb |
| 中文名稱 | 酪氨酸酶重組兔單抗 |
| 別 名 | ATN; CMM8; LB24 AB; SHEP3; SK29 AB; Tumor rejection antigen AB; TYR. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | 20C5 |
| 交叉反應(yīng) | Human |
| 產(chǎn)品應(yīng)用 | WB=1:500-1:2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 60 kDa |
| 檢測分子量 | 70-80 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human Tyrosinase: 1-300/529 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the initial and rate limiting step in the cascade of reactions leading to melanin production from tyrosine. In addition to hydroxylating tyrosine to DOPA (3,4-dihydroxyphenylalanine), also catalyzes the oxidation of DOPA to DOPA-quinone, and possibly the oxidation of DHI (5,6-dihydroxyindole) to indole-5,6 quinone. SWISS: P14679 Gene ID: 7299 酪氨酸酶( Tyrosinase) 又稱酚氧化酶、多酚氧化酶、兒茶酚氧化酶,是結(jié)構(gòu)復(fù)雜的多亞基的含銅氧化還原酶,廣泛存在于微生物、動植物及人體中。 酪氨酸酶具有獨特的雙重催化功能,是生物體內(nèi)黑色素合成的關(guān)鍵酶,與人的衰老,昆蟲的傷口愈合與發(fā)育,果蔬的褐變有密切關(guān)系。多年來,酪氨酸酶一直受到國內(nèi)外的關(guān)注,其研究涉及生物、醫(yī)學(xué)、農(nóng)學(xué)、化學(xué)、藥學(xué)等多個學(xué)科和領(lǐng)域.酪氨酸酶作為黑色素合成的關(guān)鍵酶,其異常過量表達可導(dǎo)致人體的色素沉著性疾病。 |
| 產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with Tyrosinase monoclonal antibody, unconjugated (bsm-61454R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
