| 產品編號 | bsm-61347R |
| 英文名稱 | HSP47 Recombinant Rabbit mAb |
| 中文名稱 | 熱休克蛋白-47重組兔單抗 |
| 別 名 | SERPH_HUMAN; SERPINH1; Serpin H1; 47 kDa heat shock protein; Arsenic-transactivated protein 3 (AsTP3); Cell proliferation-inducing gene 14 protein; Collagen-binding protein (Colligin); Rheumatoid arthritis-related antigen RA-A47; CBP1; CBP2; SERPINH2; PIG14; serpin family H member 1; OI10; gp46; AsTP3; PIG14; PPROM; RA-A47; |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | |
| 交叉反應 | Human,Mouse,Rat |
| 產品應用 | WB=1:500-2000,IHC-P=1:50-200,IHC-F=1:50-200,IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 46 kDa |
| 檢測分子量 | 46 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human Serpin H1: 400-418/418 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
This gene encodes a member of the serpin superfamily of serine proteinase inhibitors. The encoded protein is localized to the endoplasmic reticulum and plays a role in collagen biosynthesis as a collagen-specific molecular chaperone. Autoantibodies to the encoded protein have been found in patients with rheumatoid arthritis. SWISS: P50454 Gene ID: 871 信號傳導（Signaling Intermediates） HSP47是一類進化上高度保守的蛋白質家族.生理狀態(tài)時協(xié)助多肽或蛋白質的正確轉位、折疊和裝配,起"分子伴侶"的作用;在應激狀態(tài)下,HSP47過表達或異位表達,作為一種自身抗原被免疫系統(tǒng)識別,誘發(fā)機體的保護性免疫應答,也可作為一種信號分子,在信號轉導中發(fā)揮作用. |
| 產品圖片 |
Western blot analysis of HeLa cell lysate. Using HSP47 (bsm-61347R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
