| 產(chǎn)品編號 | bsm-61294R |
| 英文名稱 | NEDD4-2 Recombinant Rabbit mAb |
| 中文名稱 | 泛素連接酶NEDD4-2重組兔單抗 |
| 別 名 | E3 ubiquitin protein ligase NEDD4 like protein; KIAA0439; NEDD4 2; NEDD4 2c; Nedd4-2; NEDD4-2a; NEDD4b; NEDD4L; NEDL3; RSP5. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | |
| 交叉反應(yīng) | Human (predicted: Mouse,Rat) |
| 產(chǎn)品應(yīng)用 | WB=1:500-1:2000,IP=1:20-1:50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 112 kDa |
| 檢測分子量 | 130 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human NEDD4L: 920-975 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
E3 ubiquitin-protein ligase that mediates the polyubiquitination of lysine and cysteine residues on target proteins and is thereby implicated in the regulation of various signaling pathways including autophagy, innate immunity or DNA repair. SWISS: Q96PU5 Gene ID: 23327 |
| 產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with NEDD4-2 monoclonal antibody, unconjugated (bsm-61294R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
