| 產(chǎn)品編號 | bsm-61236R |
| 英文名稱 | Dysferlin Recombinant Rabbit mAb |
| 中文名稱 | Dysferlin蛋白重組兔單抗 |
| 別 名 | DYSF; Dysferlin; Fer-1-like protein 1. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | 12B8 |
| 交叉反應(yīng) | Human,Mouse |
| 產(chǎn)品應(yīng)用 | WB=1:500-1:2000,IHC-P=1:50-1:200,IHC-F=1:50-1:200,IF=1:50-1:200,ICC/IF=1:50-1:200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 237 kDa |
| 檢測分子量 | 280 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human Dysferlin: 100-200 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Key calcium ion sensor involved in the Ca2+-triggered synaptic vesicle-plasma membrane fusion. Plays a role in the sarcolemma repair mechanism of both skeletal muscle and cardiomyocytes that permits rapid resealing of membranes disrupted by mechanical stress. SWISS: O75923 Gene ID: 8291 Dysferlin是與漿膜內(nèi)環(huán)境有關(guān)的一種跨膜蛋白，與肌膜損傷的修復(fù)有關(guān). |
| 產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with Dysferlin monoclonal antibody, unconjugated (bsm-61236R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Dysferlin Monoclonal Antibody, Unconjugated (bsm-61236R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Dysferlin Monoclonal Antibody, Unconjugated (bsm-61236R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Dysferlin Monoclonal Antibody, Unconjugated (bsm-61236R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Dysferlin Monoclonal Antibody, Unconjugated (bsm-61236R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
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