| 產(chǎn)品編號 | bs-12599R-APC |
| 英文名稱1 | Rabbit Anti-PDK3/APC Conjugated antibody |
| 中文名稱 | APC標記的丙酮酸脫氫酶激酶同功酶3抗體 |
| 別 名 | [Pyruvate dehydrogenase [lipoamide] kinase isozyme 3 mitochondrial; [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 3; CMTX6; GS1-358P8.4; mitochondrial; PDK3; PDK3_HUMAN; Pyruvate dehydrogenase kinase isoenzyme 3; Pyruvate dehydrogenase kinase isoform 3; Pyruvate dehydrogenase kinase isozyme 3; Pyruvate dehydrogenase lipoamide kinase isozyme 3 mitochondrial. |
| 規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 研究領域 | 細胞生物 信號轉導 激酶和磷酸酶 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | (predicted: Human, Mouse, Rat, Dog, Pig, Horse, Rabbit, Sheep, ) |
| 產(chǎn)品應用 | ICC=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 47kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PDK3 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產(chǎn)品介紹 |
background: The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle, and thus is one of the major enzymes responsible for the regulation of glucose metabolism. The enzymatic activity of PDH is regulated by a phosphorylation/dephosphorylation cycle, and phosphorylation results in inactivation of PDH. The protein encoded by this gene is one of the three pyruvate dehydrogenase kinases that inhibits the PDH complex by phosphorylation of the E1 alpha subunit. This gene is predominantly expressed in the heart and skeletal muscles. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010] Function: Inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism. Subcellular Location: Mitochondrion matrix. Tissue Specificity: Found almost exclusively in heart and skeletal muscle. Similarity: Belongs to the PDK/BCKDK protein kinase family. Contains 1 histidine kinase domain. Database links: Entrez Gene: 5165 Human Entrez Gene: 236900 Mouse Omim: 602526 Human SwissProt: Q15120 Human SwissProt: Q922H2 Mouse Unigene: 296031 Human Unigene: 12775 Mouse Unigene: 18101 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
