產(chǎn)品編號 | bs-15364R-BF555 |
英文名稱1 | Rabbit Anti-GPR85/BF555 Conjugated antibody |
中文名稱 | BF555標記的G蛋白偶聯(lián)受體GPR85蛋白抗體 |
別 名 | GPR85; Probable G-protein coupled receptor 85; SREB; SREB2; Super conserved receptor expressed in brain 2; GPR85_HUMAN; GPCR 85. |
規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
說 明 書 | 100ul |
研究領域 | 細胞生物 免疫學 G蛋白偶聯(lián)受體 G蛋白信號 |
抗體來源 | Rabbit |
克隆類型 | Polyclonal |
交叉反應 | (predicted: Human, Mouse, Rat, Chicken, Cow, ) |
產(chǎn)品應用 | ICC=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
分 子 量 | 42kDa |
性 狀 | Lyophilized or Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human GPR85 |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol |
保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
產(chǎn)品介紹 |
background: G protein-coupled receptors (GPCRs, or GPRs) contain 7 transmembrane domains and transduce extracellular signals through heterotrimeric G proteins. GPCR GPR85 is a member of this family (subfamily Orphan-A). GPCR GPR85 has been reported to be expressed in several areas of human and rat CNS (Matsumoto et al., 2000), as well as in genital organs. ESTs have been isolated from B-cell/lung/testis, brain, testis, and embryo libraries, as well as many mouse tissue libraries. Function: Orphan receptor. Subcellular Location: Cell membrane; Multi-pass membrane protein. Tissue Specificity: Highly expressed in brain and testis. Lower levels in small intestine, placenta and spleen. In brain regions, detected in all regions tested, but somewhat lower levels in the corpus callosum, medulla and spinal cord. Similarity: Belongs to the G-protein coupled receptor 1 family. Database links: Entrez Gene: 417775 Chicken Entrez Gene: 54329 Human Entrez Gene: 64450 Mouse Entrez Gene: 100190819 Orangutan Entrez Gene: 793299 Zebrafish Omim: 605188 Human SwissProt: P60893 Human SwissProt: P60894 Mouse SwissProt: Q5RBG7 Orangutan SwissProt: Q9I919 Zebrafish Unigene: 152009 Human Unigene: 167625 Mouse Unigene: 53985 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
1、抗體溶解方法 | |
2、抗體修復方式 | |
3、常用試劑的配制 | |
4、免疫組化操作步驟 | |
5、免疫組化問題解答 | |
6、Western Blotting 操作步驟 | |
7、Western Blotting 問題解答 | |
8、關于肽鏈的設計 | |
9、多肽的溶解與保存 | |
10、酶標抗體效價測定程序 | |