| 產(chǎn)品編號 | bs-10097R-FITC |
| 英文名稱1 | Rabbit Anti-RRAGC/FITC Conjugated antibody |
| 中文名稱 | FITC標(biāo)記的RAS相關(guān)GTP結(jié)合蛋白C抗體 |
| 別 名 | RAGC; TIB929; YGR163W; GTPase interacting protein 2; GTPase-interacting protein 2; GTR2; Rag C; Rag C protein; Ras related GTP binding C; Ras-related GTP-binding protein C; RP23 29H22.4; RRAGC; RRAGC_HUMAN. |
| 規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 研究領(lǐng)域 | 細(xì)胞生物 染色質(zhì)和核信號 信號轉(zhuǎn)導(dǎo) G蛋白信號 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Mouse, (predicted: Human, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep, ) |
| 產(chǎn)品應(yīng)用 | Flow-Cyt=1ug/Test ICC=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 44kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human RRAGC |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產(chǎn)品介紹 |
background: This gene encodes a member of the GTR/RAG GTP-binding protein family. The encoded protein is a monomeric guanine nucleotide-binding protein which forms a heterodimer with RRAGA and RRAGB and is primarily localized to the cytoplasm. The encoded protein promotes intracellular localization of the mTOR complex. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2012]. Function: Has guanine nucleotide-binding activity but weak intrinsic GTPase activity. Probably required for the amino acid-induced relocalization of mTORC1 to the lysosomes and its subsequent activation by the GTPase RHEB. This is a crucial step in the activation of the TOR signaling cascade by amino acids. Subunit: Forms a heterodimer with RRAGA in a sequence-independent manner. Binds GTP. Interacts with NOL8 and RRAGB. Subcellular Location: Cytoplasm. Nucleus. Lysosome. Note=Predominantly cytoplasmic. May shuttle between the cytoplasm and nucleus, depending on the bound nucleotide state of associated RRAGA. Similarity: Belongs to the GTR/RAG GTP-binding protein family. Database links: Entrez Gene: 64121 Human Entrez Gene: 54170 Mouse Omim: 608267 Human SwissProt: Q9HB90 Human SwissProt: Q99K70 Mouse Unigene: 532461 Human Unigene: 220922 Mouse Unigene: 473825 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產(chǎn)品圖片 |
Blank control: Mouse spleen.
Primary Antibody (green line): Rabbit Anti-RRAGC antibody (bs-10097R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.
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