產(chǎn)品編號 | bs-14301R-HRP |
英文名稱1 | Rabbit Anti-DGKZ/HRP Conjugated antibody |
中文名稱 | 辣根過氧化物酶標記的甘油二酯激酶ζ/DGK-ζ抗體 |
別 名 | DAG kinase zeta; DAGK5; DAGK5 PEN; DAGK6; DGK ZETA; DGK-zeta; Dgkz; DGKZ_HUMAN; Diacylglycerol kinase zeta; Diglyceride kinase zeta; hDGKzeta. |
規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
說 明 書 | 100ul |
研究領域 | 心血管 細胞生物 信號轉(zhuǎn)導 新陳代謝 |
抗體來源 | Rabbit |
克隆類型 | Polyclonal |
交叉反應 | Rat, (predicted: Human, Mouse, Pig, Cow, Guinea Pig, ) |
產(chǎn)品應用 | WB=1:500-2000 ELISA=1:100-1000 IHC-P=1:50-200 IHC-F=1:50-200 ICC=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
分 子 量 | 124kDa |
性 狀 | Lyophilized or Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human DGKZ |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
產(chǎn)品介紹 |
background: The protein encoded by this gene belongs to the eukaryotic diacylglycerol kinase family. It may attenuate protein kinase C activity by regulating diacylglycerol levels in intracellular signaling cascade and signal transduction. Alternative splicing occurs at this locus and multiple transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Nov 2010] Function: Displays a strong preference for 1,2-diacylglycerols over 1,3-diacylglycerols, but lacks substrate specificity among molecular species of long chain diacylglycerols. Isoform 2 but not isoform 1 regulates RASGRP1 activity. Subcellular Location: Cytoplasm. Nucleus. Cell membrane. Tissue Specificity: Highest levels in brain, and substantial levels in skeletal muscle, heart, and pancreas. Isoform 1 is predominantly expressed in muscle. Post-translational modifications: Phosphorylation of the MARCKS homology domain by PKC reduces nuclear accumulation of DGK-zeta. Similarity: Belongs to the eukaryotic diacylglycerol kinase family. Contains 2 ANK repeats. Contains 1 DAGKc domain. Contains 2 phorbol-ester/DAG-type zinc fingers. Database links: Entrez Gene: 8525 Human Entrez Gene: 423197 Chicken Entrez Gene: 100050499 Horse Entrez Gene: 104418 Mouse Omim: 601441 Human SwissProt: Q13574 Human SwissProt: Q80UP3 Mouse Unigene: 502461 Human Unigene: 314923 Mouse Unigene: 11208 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
1、抗體溶解方法 | |
2、抗體修復方式 | |
3、常用試劑的配制 | |
4、免疫組化操作步驟 | |
5、免疫組化問題解答 | |
6、Western Blotting 操作步驟 | |
7、Western Blotting 問題解答 | |
8、關于肽鏈的設計 | |
9、多肽的溶解與保存 | |
10、酶標抗體效價測定程序 | |