| 產品編號 | bs-6795R-BF488 |
| 英文名稱1 | Rabbit Anti-CHAC1/BF488 Conjugated antibody |
| 中文名稱 | BF488標記的陽離子轉運調控樣蛋白1抗體 |
| 別 名 | Cation transport regulator like protein 1; Cation transport regulator-like protein 1; ChaC; chac1; CHAC1_HUMAN. |
| 規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 研究領域 | 細胞生物 信號轉導 細胞凋亡 轉錄調節(jié)因子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human, Mouse, Rat, (predicted: Dog, Pig, Cow, Rabbit, ) |
| 產品應用 | Flow-Cyt=2ug/Test IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 29kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CHAC1 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產品介紹 |
background: Pro-apoptotic component of the unfolded protein response pathway. May mediate the pro-apoptotic effects of the ATF4-ATF3-DDIT3/CHOP cascade. Function: Pro-apoptotic component of the unfolded protein response pathway. May mediate the pro-apoptotic effects of the ATF4-ATF3-DDIT3/CHOP cascade. Subcellular Location: Cytoplasm, cytosol. Similarity: elongs to the chaC family. Database links: Entrez Gene: 79094 Human Entrez Gene: 69065 Mouse GenBank: NP_077016 Human SwissProt: Q9BUX1 Human SwissProt: Q8R3J5 Mouse Unigene: 155569 Human Unigene: 35083 Mouse Unigene: 23367 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產品圖片 |
Blank control: U2OS.
Primary Antibody (green line): Rabbit Anti-CHAC1 antibody (bs-6795R-BF488)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
