| 產(chǎn)品編號 | bs-1226R-AP |
| 英文名稱1 | Rabbit Anti-GPA33/AP Conjugated antibody |
| 中文名稱 | 堿性磷酸酶（AP）標記的糖蛋白33抗體 |
| 別 名 | Cell surface A33 antigen precursor; A33; Cell surface A33 antigen; Gpa33; GPA33_HUMAN; Transmembrane glycoprotein A33; Glycoprotein A33 (transmembrane); MGC129986; MGC129987; glycoprotein A33 transmembrane. |
| 規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 研究領(lǐng)域 | 腫瘤 免疫學(xué) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human, Mouse, (predicted: Rat, ) |
| 產(chǎn)品應(yīng)用 | WB=1:50-200 IHC-P=1:50-200 IHC-F=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 33kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GPA33 C-terminus |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產(chǎn)品介紹 |
background: The glycoprotein encoded by this gene is a cell surface antigen that is expressed in greater than 95% of human colon cancers. The open reading frame encodes a 319-amino acid polypeptide having a putative secretory signal sequence and 3 potential glycosylation sites. The predicted mature protein has a 213-amino acid extracellular region, a single transmembrane domain, and a 62-amino acid intracellular tail. The sequence of the extracellular region contains 2 domains characteristic of the CD2 subgroup of the immunoglobulin (Ig) superfamily. [provided by RefSeq, Jul 2008] Function: May play a role in cell-cell recognition and signaling. Subcellular Location: Membrane; Single-pass type I membrane protein. Tissue Specificity: Expressed in normal gastrointestinal epithelium and in 95% of colon cancers. Post-translational modifications: N-glycosylated, contains approximately 8 kDa of N-linked carbohydrate. Palmitoylated. Similarity: Contains 1 Ig-like C2-type (immunoglobulin-like) domain. Contains 1 Ig-like V-type (immunoglobulin-like) domain. Database links: Entrez Gene: 10223 Human Omim: 602171 Human SwissProt: Q99795 Human Unigene: 651244 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. GPA33是胃腸道上皮細胞特異性表達的跨膜糖蛋白,在95％以上的大腸癌、63％的胃癌和50％的胰腺癌中表達。 |
| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
