| 產品編號 | bs-2370R-FITC |
| 英文名稱1 | Rabbit Anti-FABP5/FITC Conjugated antibody |
| 中文名稱 | FITC標記的脂肪酸結合蛋白5/上皮細胞型抗體 |
| 別 名 | epidermal; Fatty Acid Binding Protein 5; CFABP; Cutaneous fatty acid binding protein; DA11; Differentiation associated lipid binding protein LP2; E-FABP; EFABP; Epidermal-type fatty acid-binding protein; FABP5; FABP5_HUMAN; Fatty acid binding protein epidermal; Fatty acid-binding protein 5; Fatty acid-binding protein; Keratinocyte lipid binding protein; PA-FABP; PAFABP; Psoriasis associated fatty acid binding protein homolog; Psoriasis-associated fatty acid-binding protein homolog. |
| 規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 研究領域 | 細胞生物 免疫學 結合蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse, (predicted: Human, Rat, Pig, Horse, ) |
| 產品應用 | Flow-Cyt=3ug/Test IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 15kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Fatty Acid Binding Protein 5 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產品介紹 |
background: This gene encodes the fatty acid binding protein found in epidermal cells, and was first identified as being upregulated in psoriasis tissue. Fatty acid binding proteins are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. FABPs may play roles in fatty acid uptake, transport, and metabolism. Polymorphisms in this gene are associated with type 2 diabetes. The human genome contains many pseudogenes similar to this locus.[provided by RefSeq, Feb 2011]. Subcellular Location: Cytoplasm. Tissue Specificity: Keratinocytes; highly expressed in psoriatic skin. Similarity: Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family. Database links: Entrez Gene: 2171 Human Omim: 605168 Human SwissProt: Q01469 Human Unigene: 408061 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產品圖片 |
Blank control (Black line): U937(Black).
Primary Antibody (green line): Rabbit Anti-FABP5/FITC antibody (bs-2370R-FITC)
Dilution: 3μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG-FITC .
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
