產(chǎn)品編號(hào) | bs-2302R-PE |
英文名稱1 | Rabbit Anti-Catalase/PE Conjugated antibody |
中文名稱 | PE標(biāo)記的過氧化氫酶抗體 |
別 名 | Cas1; CAT; Cs1; MGC138422; MGC138424. |
規(guī)格價(jià)格 | 100ul/2980元 購買 大包裝/詢價(jià) |
說 明 書 | 100ul |
研究領(lǐng)域 | 細(xì)胞生物 免疫學(xué) 發(fā)育生物學(xué) 激酶和磷酸酶 |
抗體來源 | Rabbit |
克隆類型 | Polyclonal |
交叉反應(yīng) | (predicted: Pig, ) |
產(chǎn)品應(yīng)用 | IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
分 子 量 | 58kDa |
性 狀 | Lyophilized or Liquid |
濃 度 | 1mg/ml |
免 疫 原 | Full length plasma protein from pig |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
儲(chǔ) 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
產(chǎn)品介紹 |
background: Catalase is the classical marker for peroxisomes and is the most abundant protein within peroxisomes. It is found in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Function: Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells. Subunit: Homotetramer. Subcellular Location: Peroxisome. Post-translational modifications: The N-terminus is blocked. DISEASE: Defects in CAT are the cause of acatalasemia (ACATLAS) [MIM:614097]. A metabolic disorder characterized by absence of catalase activity in red cells and is often associated with ulcerating oral lesions. Similarity: Belongs to the catalase family. Database links: Entrez Gene: 847 Human Entrez Gene: 12359 Mouse Omim: 115500 Human SwissProt: P04040 Human SwissProt: P24270 Mouse Unigene: 502302 Human Unigene: 4215 Mouse Unigene: 3001 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 過氧化氫酶是催化過氧化氫分解成氧和水的酶,存在于細(xì)胞的過氧化物體內(nèi)。過氧化氫酶是過氧化物酶體的標(biāo)志酶, 約占過氧化物酶體酶總量的40%。過氧化氫酶存在于所有已知的動(dòng)物的各個(gè)組織中,特別在肝臟中以高濃度存在。過氧化氫酶在食品工業(yè)中被用于除去用于制造奶酪的牛奶中的過氧化氫。過氧化氫酶也被用于食品包裝,防止食物被氧化。 ????過氧化氫酶(CAT)是一種酶類清除劑,又稱為觸酶,是以鐵卟啉為輔基的結(jié)合酶。它可促使H2O2分解為分子氧和水,清除體內(nèi)的過氧化氫,從而使細(xì)胞免于遭受H2O2的毒害,是生物防御體系的關(guān)鍵酶之一。CAT作用于過氧化氫的機(jī)理實(shí)質(zhì)上是H2O2的歧化,必須有兩個(gè)H2O2先后與CAT相遇且碰撞在活性中心上,才能發(fā)生反應(yīng)。H2O2濃度越高,分解速度越快。 ????幾乎所有的生物機(jī)體都存在過氧化氫酶。其普遍存在于能呼吸的生物體內(nèi),主要存在于植物的葉綠體、線粒體、內(nèi)質(zhì)網(wǎng)、動(dòng)物的肝和紅細(xì)胞中,其酶促活性為機(jī)體提供了抗氧化防御機(jī)理。 CAT是紅血素酶,不同的來源有不同的結(jié)構(gòu)。在不同的組織中其活性水平高低不同。過氧化氫在肝臟中分解速度比在腦或心臟等器官快,就是因?yàn)楦沃械腃AT含量水平高。 ????過氧化氫酶在食品工業(yè)中被用于除去用于制造奶酪的牛奶中的過氧化氫。過氧化氫酶也被用于食品包裝,防止食物被氧化。在紡織工業(yè)中,過氧化氫酶被用于除去紡織物上的過氧化氫,以保證成品是不含過氧化物的。它還被用在隱形眼鏡的清潔上:眼鏡在含有過氧化氫的清潔劑中浸泡后,使用前再用過氧化氫酶除去殘留的過氧化氫。近年來,過氧化氫酶開始使用在美容業(yè)中。一些面部護(hù)理中加入了該酶和過氧化氫,目的是增加表皮上層的細(xì)胞氧量。 |