| 產(chǎn)品編號 | bs-3155R-PE |
| 英文名稱1 | Rabbit Anti-Phospho-GCN2 (Thr899)/PE Conjugated antibody |
| 中文名稱 | PE標記的磷酸化蛋白激酶GCN2蛋白抗體 |
| 別 名 | GCN2 (phospho T899); p-GCN2 (phospho T899); EIF2AK4; Eukaryotic Translation Initiation Factor 2-alpha kinase 4; GCN2-like protein; KIAA1338; E2AK4_HUMAN; Eif2ak4; Eukaryotic Translation Initiation Factor 2 alpha kinase 4; GCN2; GCN2 eIF2alpha kinase; GCN2-like protein; MGCN2. |
| 規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 產(chǎn)品類型 | 磷酸化抗體 |
| 研究領(lǐng)域 | 腫瘤 細胞生物 染色質(zhì)和核信號 神經(jīng)生物學(xué) 信號轉(zhuǎn)導(dǎo) 激酶和磷酸酶 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human, Mouse, (predicted: Rat, Dog, Cow, Horse, ) |
| 產(chǎn)品應(yīng)用 | Flow-Cyt=2ug/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 189kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human GCN2 around the phosphorylation site of Thr899 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300. |
| 保存條件 | Store at 2-8℃ for one year. Avoid repeated freeze/thaw cycles. |
| 產(chǎn)品介紹 |
background: GCN2 belongs to a family of kinases that phosphorylate the alpha subunit of eukaryotic translation initiation factor 2 to downregulate protein synthesis in response to varied cellular stresses. Function: Can phosphorylate the alpha subunit of EIF2 and may mediate translational control. Tissue Specificity: Widely expressed. Post-translational modifications: Autophosphorylated on threonine residues. Similarity: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily. Contains 2 protein kinase domains. Contains 1 RWD domain. Database links: Entrez Gene: 440275 Human Entrez Gene: 27103 Mouse Omim: 609280 Human SwissProt: Q9P2K8 Human SwissProt: Q9QZ05 Mouse Unigene: 656673 Human Unigene: 217616 Mouse Unigene: 8333 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. GCN2蛋白的作用；可能是將短期記憶變成長期記憶的一個主控調(diào)節(jié)因子， GCN2蛋白在長期記憶形成中功能的發(fā)現(xiàn)將可能幫助研究人員開發(fā)出提高記憶力喪失的病人提高記憶力。 |
| 產(chǎn)品圖片 |
Mouse macrophage cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Phospho-GCN2 (Thr899) Antibody(bs-3155R-PE)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (purple),blank(orange).Shanghai Institute of Nutrition and Health/Professor Guo’s laboratory
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
