| 產(chǎn)品編號 | bs-2247R-Bio |
| 英文名稱1 | Rabbit Anti-AHA3/Biotin Conjugated antibody |
| 中文名稱 | 生物素標記的AHA3抗體（擬南芥） |
| 別 名 | ATPase/hydrogen-exporting ATPase, phosphorylative mechanism; member of Plasma membrane H+-ATPase family; PMA3_ARATH. |
| 規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 產(chǎn)品類型 | 植物抗體 |
| 研究領(lǐng)域 | 細胞生物 免疫學 轉(zhuǎn)錄調(diào)節(jié)因子 植物 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | (predicted: Arabidopsis Thaliana, ) |
| 產(chǎn)品應(yīng)用 | ELISA=1:100-1000 IHC-P=1:50-200 IHC-F=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 104kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from Arabidopsis thaliana (thale cress) AHA3 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產(chǎn)品介紹 |
Function: The plasma membrane H(+) ATPase of plants and fungi generates a proton gradient that drives the active transport of nutrients by H(+)-symport. The resulting external acidification and/or internal alkinization may mediate growth responses. Subunit: Binds to 14-3-3 proteins. The binding is induced by phosphorylation of Thr-948. Binding to 14-3-3 proteins activates the H(+)-ATPase. Subcellular Location: Cell membrane; Multi-pass membrane proteinc(Probable). Tissue Specificity: Found predominantly in phloem cells of leaves, stems, roots and flowers. Post-translational modifications: Phosphorylation level varies significantly during early response to bacterial elicitor (e.g. fusicoccin FC). Similarity: Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIIA subfamily. Database links: Entrez Gene: 835841 Arabidopsis thaliana SwissProt: P20431 Arabidopsis thaliana Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
