| 產(chǎn)品編號(hào) | bs-1687R-AP |
| 英文名稱1 | Rabbit Anti-DAPK1/AP Conjugated antibody |
| 中文名稱 | 堿性磷酸酶（AP）標(biāo)記的凋亡相關(guān)蛋白激酶1抗體 |
| 別 名 | DAP Kinase 1; DAPK1; DAK1; DAP K1; DAPK 1; DAPK; DAPK1; Death Associated Protein Kinase 1; Death Associated Protein Kinase 1; Death-associated protein kinase 1; DKFZp781I035; DAPK1_HUMAN. |
| 規(guī)格價(jià)格 | 100ul/2980元 購(gòu)買 大包裝/詢價(jià) |
| 說(shuō) 明 書 | 100ul |
| 研究領(lǐng)域 | 腫瘤 細(xì)胞生物 信號(hào)轉(zhuǎn)導(dǎo) 細(xì)胞凋亡 激酶和磷酸酶 |
| 抗體來(lái)源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | (predicted: Human, Mouse, Rat, ) |
| 產(chǎn)品應(yīng)用 | WB=1:50-200 IHC-P=1:50-200 IHC-F=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 160kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human DAPK1 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲(chǔ) 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產(chǎn)品介紹 |
background: DAPK1 expression is frequently lost in human carcinomas and B-cell leukemia, and lower levels of expression correlates with high rates of metastasis. The loss of DAPK expression provides a link between suppression of apoptosis and metastasis. DAPK1 is thought be involved in an early apoptotic checkpoint which eliminates premalignant cells from cancer formation. Studies in bladder cancer patients have also shown that hypermethylation of DAPK1 correlates to high recurrence rates and thus DAPK1 may be used as a prognostic marker. DAPK1 is also reportedly a molecular regulator of neuronal death in epilepsy. Function: Calcium/calmodulin-dependent serine/threonine kinase which acts as a positive regulator of apoptosis. Subunit: Interacts with KLHL20. Interacts (via death domain) with MAPK1 and MAPK3. Interacts with MAP1B (via N-terminus). Interacts (via death domain) with UNC5B (via death domain). Interacts with PRKD1 in an oxidative stress-regulated manner. Interacts with PIN1, PDCD6, BECN1, GRINB, TSC2 and STX1A. Interacts (via kinase domain) with DAPK3 (via kinase domain). Subcellular Location: Cytoplasm. Colocalizes with the actin filament system. Tissue Specificity: Isoform 2 is expressed in normal intestinal tissue as well as in colorectal carcinomas. Post-translational modifications: Ubiquitinated by the BCR(KLHL20) E3 ubiquitin ligase complex, leading to its degradation by the proteasome. Similarity: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily. Contains 10 ANK repeats. Contains 1 death domain. Contains 1 protein kinase domain. Database links: Entrez Gene: 1612 Human Entrez Gene: 69635 Mouse Omim: 600831 Human SwissProt: P53355 Human SwissProt: Q80YE7 Mouse Unigene: 380277 Human Unigene: 693441 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 凋亡相關(guān)蛋白激酶1(DAPK1)又稱：一般性白血病的基因，也是一種腫瘤抑制基因，廣泛存在于各種細(xì)胞的凋亡系統(tǒng)中，該基因正常情況下有助于觸發(fā)細(xì)胞在發(fā)生癌變前就死亡。但異常的DNA甲基化作用會(huì)關(guān)閉控制細(xì)胞生長(zhǎng)的DAPK1基因，從而造成腫瘤生長(zhǎng)。 |
