| 產(chǎn)品編號 | bs-34067R |
| 英文名稱 | [KO驗證抗體] Rabbit Anti-LAMB1 antibody |
| 中文名稱 | 層粘連蛋白β1抗體 |
| 別 名 | LAMB1; Laminin B1 chain; Laminin subunit beta 1; Laminin-1 subunit beta; Laminin-10 subunit beta; Laminin-12 subunit beta; Laminin-2 subunit beta; Laminin-6 subunit beta; Laminin-8 subunit beta;LAMB1_HUMAN. |
| 研究領(lǐng)域 | 腫瘤 細胞生物 免疫學 神經(jīng)生物學 信號轉(zhuǎn)導 細胞凋亡 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 198kDa |
| 細胞定位 | 細胞膜 細胞外基質(zhì) 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant human laminin: 1467-1786 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
laminins are the major noncollagenous components of basement membranes that mediate cell adhesion, growth migration, and differentiation; the laminin-type epidermal growth factor-like module occurs in tandem arrays; the domain contains 4 disulfide bonds (loops a-d) the first three resemble epidermal growth factor (EGF); the number of copies of this domain in the different forms of laminins is highly variable ranging from 3 up to 22 copies. Function: Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Subcellular Location: Secreted > extracellular space > extracellular matrix > basement membrane. Tissue Specificity: Broadly expressed in: skin, heart, lung, and the reproductive tracts. Similarity: Contains 11 laminin EGF-like domains. Contains 1 laminin IV type A domain. Contains 1 laminin N-terminal domain. SWISS: P07942 Gene ID: 3912 Database links: Entrez Gene: 10319 Human Entrez Gene: 284217 Human Entrez Gene: 3908 Human Entrez Gene: 3912 Human Entrez Gene: 3913 Human Entrez Gene: 3915 Human Entrez Gene: 3918 Human Entrez Gene: 16772 Mouse Omim: 150320 Human SwissProt: P07942 Human SwissProt: P24043 Human SwissProt: P25391 Human SwissProt: P55268 Human SwissProt: Q9Y6N6 Human SwissProt: P19137 Mouse SwissProt: Q9R0B6 Mouse Unigene: 201805 Human Unigene: 270364 Human Unigene: 302362 Mouse 層粘連蛋白主要存在于基膜(basal lamina)結(jié)構(gòu)中，是基膜所特有的非膠原糖蛋白。 層粘連蛋白在細胞發(fā)育過程中刺激細胞粘著、細胞運動。LN能夠刺激胚胎中神經(jīng)軸的生長,并促進成年動物的神經(jīng)損傷后重生長和再生。如同纖粘連蛋白,細胞外的LN能夠影響細胞的生長、遷移和分化。LN在原生殖細胞的遷移中起關(guān)鍵作用。 層粘連蛋白是細胞外基底膜的主要組成部分，分布于基底膜和細胞外基質(zhì)內(nèi)、成纖維細胞、內(nèi)皮細胞和平滑肌細胞分泌層粘連蛋白。腫瘤的浸潤和轉(zhuǎn)移與其遭到破壞相關(guān)。該抗體可用于標記基底膜，有助于觀察腫瘤周圍基底膜的分布及其改變，間接提示該腫瘤的分化程度和浸潤情況。 |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Heart (Mouse) Lysate at 40 ug
Lane 2: Skin (Mouse) Lysate at 40 ug
Lane 3: Placenta (Mouse) Lysate at 40 ug
Lane 4: U2os (Human) Cell Lysate at 30 ug
Lane 5: Huvec (Human) Cell Lysate at 30 ug
Lane 6: HepG2 (Human) Cell Lysate at 30 ug
Lane 7: SH-SY5Y (Human) Cell Lysate at 30 ug
Lane 8: A549 (Human) Cell Lysate at 30 ug
Lane 9: U251 (Human) Cell Lysate at 30 ug
Primary: Anti-laminin (bs-34067R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 220-240 kD
Observed band size: 240 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
