| 產(chǎn)品編號 | bsm-54329R |
| 英文名稱 | Rabbit Anti-Cubilin antibody |
| 中文名稱 | 內(nèi)皮因子維生素B12受體重組兔單抗 |
| 別 名 | 460 kDa receptor; cubilin (intrinsic factor-cobalamin receptor); Cubilin precursor; CUBN; IFCR; Intestinal intrinsic factor receptor; intrinsic factor B12-receptor; intrinsic factor-cobalamin receptor; intrinsic factor-vitamin B12 receptor; megaloblastic anemia 1; MGA1. |
| 研究領(lǐng)域 | 腫瘤 細(xì)胞生物 免疫學(xué) 染色質(zhì)和核信號 轉(zhuǎn)錄調(diào)節(jié)因子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | 7B12 |
| 交叉反應(yīng) | Human |
| 產(chǎn)品應(yīng)用 | WB=1:500,IHC-P=1:100-500,IHC-F=1:400-800,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 395kDa |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant human Cubilin protein |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.02% Proclin300. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Cubilin is an endocytic receptor that lacks a classical transmembrane region. It is a multidomain receptor that contains an amino terminal 110 residue segment followed by 8 epidermal growth factor (EGF)-like repeats and a contiguous stretch of 27 CUB domains. Cubilin acts as a receptor for intrinsic factor-vitamin B12 complexes. Cubilin is located within the epithelium of intestine and kidney. Cubilin co-localizes with and binds to Megalin, a 600kDa member of the LDL receptor family that is required for the internalization of cubilin-bound ligands, such as vitamin B12, apolipoprotein A1 and Albumin. Megalin specifically binds to cubilin in the amino terminal region that contains the EGF-like repeats and CUB domains 1 and 2. Mutations in Cubilin may play a role in autosomal recessive megaloblastic anemia also known as Imerslund-Grasbeck’s disease, which causes intestinal malabsorption of vitamin B12. SWISS: O60494 Gene ID: 8029 |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cubilin) Monoclonal Antibody, Unconjugated (bsm-54329R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human kidney carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cubilin) Monoclonal Antibody, Unconjugated (bsm-54329R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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