產品編號 | bsm-54190R |
英文名稱 | Rabbit Anti-Citrate synthase antibody |
中文名稱 | 檸檬酸合成酶CISY重組兔單抗 |
別 名 | CISY_HUMAN; Citrate synthase, mitochondrial; Cs; EC 2.3.3.1; Citrate (Si)-synthase. 檸檬酸合酶CS |
研究領域 | 腫瘤 細胞生物 信號轉導 新陳代謝 |
抗體來源 | Rabbit |
克隆類型 | Recombinant |
克 隆 號 | 3C3 |
交叉反應 | Human,Mouse,Rat |
產品應用 | WB=1:200-1000,IHC-P=1:100-500,IHC-F=1:400-800,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理論分子量 | 52kDa |
細胞定位 | 細胞漿 |
性 狀 | Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human Citrate synthase |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
緩 沖 液 | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.02% Proclin300. |
保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
產品介紹 |
The protein encoded by this gene is a Krebs tricarboxylic acid cycle enzyme that catalyzes the synthesis of citrate from oxaloacetate and acetyl coenzyme A. The enzyme is found in nearly all cells capable of oxidative metablism. This protein is nuclear encoded and transported into the mitochondrial matrix, where the mature form is found. [provided by RefSeq, Jul 2008] SWISS: O75390 Gene ID: 1431 |
產品圖片 |
Sample:
Lane 1: Mouse Heart tissue lysates
Lane 2: Mouse Muscle tissue lysates
Lane 3: Mouse Cerebrum tissue lysates
Lane 4: Mouse Spleen tissue lysates
Lane 5: Rat Heart tissue lysates
Lane 6: Rat Muscle tissue lysates
Lane 7: Rat Cerebrum tissue lysates
Lane 8: Rat Spleen tissue lysates
Lane 9: Human 293T cell lysates
Lane 10: Human HeLa cell lysates
Lane 11: Human HL-60 cell lysates
Lane 12: Human HUVEC cell lysates
Lane 13: Human HepG2 cell lysates
Primary: Anti-Citrate synthase (bsm-54190R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52 kDa
Observed band size: 45 kDa
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1、抗體溶解方法 | |
2、抗體修復方式 | |
3、常用試劑的配制 | |
4、免疫組化操作步驟 | |
5、免疫組化問題解答 | |
6、Western Blotting 操作步驟 | |
7、Western Blotting 問題解答 | |
8、關于肽鏈的設計 | |
9、多肽的溶解與保存 | |
10、酶標抗體效價測定程序 | |